[Histonet] Luciferase IHC

[O'Donnell, Bill]

Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you)
(It's only Wednesday, what'll Friday look like?)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, November 01, 2011 7:01 PM
To: Randolph-Habecker, Julie; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC

You have to soak it in holy water, then all H311 will break out,,,,
 
Sorry, I couldnt resist. 
 
 


________________________________
From: "Randolph-Habecker, Julie" <jhabecke <@t> fhcrc.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, November 1, 2011 6:49 PM
Subject: [Histonet] Luciferase IHC

Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use.



Thanks!!



Julie



Julie Randolph-Habecker, Ph.D.

Director, Experimental Histopathology Shared Resources

Fred Hutchinson Cancer Research Center

1100 Fairview Ave N, DE-360

Seattle WA 98109-1024

Tel: 206-667-6119

Fax: 206-667-6845

jhabecke <@t> fhcrc.org



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