[Histonet] Problems with Frozen tissues

[Daniela Bodemer]

Our tissues are frozen in OCT. I use Super Frost Plus slides from Thermo Scientific and store the sections in the fridge until I need them. After leaving them out on the bench for an hour I start my H&E with 1 min running water and so on. I rarely lose sections with these slides.

Daniela

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, 1 November 2011 3:02 AM
To: Igor Deyneko; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Problems with Frozen tissues

Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. 
 
Kim


________________________________
From: Igor Deyneko <igor.deyneko <@t> gmail.com>
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Monday, October 31, 2011 9:56 AM
Subject: [Histonet] Problems with Frozen tissues

I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief?
Thank you in advance.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
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