[Histonet] Alcian blue quistion

[mohamed abd el razik]

hi every body
i have a confusing quistion reagrding Alcian blue pH 1 & 2.5 stains.
suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell only react to pH 2.5 althogh the other non reacted cells possess acidic sulfated type?? on other word i need to know for what type of mucins alcian B 2.5 react with?
 
thanx all