[Histonet] Help me again please

[sgoebel <@t> mirnarx.com]

What dilution do you guys use on a rabbit polyclonal Ki-67 antibody...I
just did 1/500 and got a crap ton of noise in the areas where the
positive staining was.  There are definite negative "rivers" flowing
through the samples, but even the tonsil control has this same fuzziness
to it.  Any suggestions?

Thanks

PS-The antibody is Abcam

 

Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912