[Histonet] Coronavirus antibody

Jan Shivers shive003 <@t> umn.edu
Tue May 24 12:14:04 CDT 2011


We have successfully stained ferret tissue using an antibody to Feline 
Infectious Peritonitis Virus (which also cross-reacts with swine 
Transmissible Gastroenteritis Virus).  We buy our antibody from Custom 
Monoclonals, Inc. and use heat-induced epitope retrieval (pressure cooker 
and target retrieval solution).  If you would like more details than this, I 
can send an uncontrolled copy of our SOP to you.

Best regards,
Jan Shivers
Senior Scientist
IHC/Histology/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
shive003 <@t> umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)

----- Original Message ----- 
From: "Chakib Boussahmain" <chak_bou <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, May 24, 2011 11:34 AM
Subject: [Histonet] Coronavirus antibody

> Hi Histonetters,
> We are interesting in doing immunostain on ferret tissues that have been 
> fixed in formalin and embedded in paraffin. I am just wondering if anyone 
> used this antibody in their lab, if so, can you please share with us your 
> staining protocol? antigen retrieval protocol as well?
> Your help will be very much appreciated.
> Thank you
> Chakib HTL(ASCP)
> Histology lab
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

More information about the Histonet mailing list