[Histonet] Coronavirus antibody

[Jan Shivers]

Hello,

We have successfully stained ferret tissue using an antibody to Feline 
Infectious Peritonitis Virus (which also cross-reacts with swine 
Transmissible Gastroenteritis Virus).  We buy our antibody from Custom 
Monoclonals, Inc. and use heat-induced epitope retrieval (pressure cooker 
and target retrieval solution).  If you would like more details than this, I 
can send an uncontrolled copy of our SOP to you.

Best regards,
Jan Shivers
Senior Scientist
IHC/Histology/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Chakib Boussahmain" <chak_bou <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, May 24, 2011 11:34 AM
Subject: [Histonet] Coronavirus antibody


> Hi Histonetters,
> We are interesting in doing immunostain on ferret tissues that have been 
> fixed in formalin and embedded in paraffin. I am just wondering if anyone 
> used this antibody in their lab, if so, can you please share with us your 
> staining protocol? antigen retrieval protocol as well?
> Your help will be very much appreciated.
> Thank you
> Chakib HTL(ASCP)
> Histology lab
> MIT
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