[Histonet] Re: Histonet Digest, Vol 90, Issue 22 2. FW: How to remove DAB to restain with DAB (Margaryan, Naira)

[Keith Mikoff]

Hi Naira,

The short answer is no, it can't be done.  

Unless there is a way to release the antibody from the binding site without 
damaging the binding site, it can't be done.  That said, somehow it should be 
possible to find a reproduce-able fix for this kind of issue, by either mending 
the binding site, or enhancing the activation of the already applied antibody 
complex and/or  chromogen.  Some really cool tricks if practical or possible.


It seems like it should, maybe with the application of an mild acidic or basic 
wash... with just the right reagent, the right amount, right application and at 
the right pH.  Something to think about.

Keith M. Mikoff, HTL (ASCP)

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Message: 2
Date: Thu, 19 May 2011 11:55:14 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] FW: How to remove DAB to restain with DAB
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Hello,

I would like to repeat my DAB staining on the same slide with DAB I run before. 
I know that "acid alcohol" will remove hematoxylin but how to remove DAB?
I appreciate to any suggestion.

Thanks in advance,
Naira



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End of Histonet Digest, Vol 90, Issue 22
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