[Histonet] Alizarin Red Troubleshooting

[Silvina Molinuevo]

Dear Amanda, if you have the staining solution well prepared (the colour must be like dark iodide solutions, I recomend you to check ammonia solutions) the problem should be that you are loosening your calcium deposits. May be you have sth wrong or may be some EDTA contamination in the fixative or hydrating and dehydrating solutions.
Best regards,



Dr María Silvina Molinuevo
Grupo de Investigacion en 
Osteopatias y Metabolismo Mineral
Departamento de Ciencias Biologicas
Facultad de Ciencias Exactas
Universidad Nacional de La Plata
47 y 115
(1900)La Plata
Argentina
www.biol.unlp.edu.ar/giomm
e-mail: silvina.molinuevo <@t> bigfoot.com