[Histonet] IHC pos. & neg. control question

[Sebree Linda A]

That's a new one on me!   


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question

I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had
no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we
get
one slide of patient tissue, then we will use the pos. and neg. cont.
from
the same block but typically it's the pt. tissue that is used for the
neg.
control. Thanks for your guidance. 

 

Email: 

"I received slides on sentinel lymph node biopsies with a positive
control
on the same slide as the breast tissue, but the negative control was
just
the patient's lymph node and did not have the corresponding section used
for
the positive control.  The patient's own tissue cannot be used as a
negative
control.  The tissue that stained positively must serve as the negative
control without the antibody.  This is critical and you need to correct
that
immediately."

 

 

Curt 

 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet