[Histonet] RE: Ventana E-cadherin problem

Wed May 18 10:35:09 CDT 2011

We treat certain antibodies (including E-Cad, and many other breast markers) by putting the slides into formalin for 15 minutes after baking.  Then, rinse in tap and put on the machine.  It helps keep tissue on the slides.  Also, as an aside, I heard directly from someone who works for Erie (who makes superfrost plus slides)that you cannot remove the positive charge from the slide, no matter how many times you dip it in water - as long as it's plain distilled water, and doesn't have any additives in it (such as Sta-on).  That being said, we had many issues with bad lots of slides where the positive charge was uneven, resulting in poor staining more often than with tissue adhesion.

Clare

Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, May 18, 2011 11:27 AM
To: Kuhnla, Melissa; Walter Benton; Dessoye, Michael J; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Ventana E-cadherin problem

We've always had problems with the tissue falling off on the E-Cad
(Ventana).  Normally, we put the patient tissue on the same slide as the
control tissue - but for the E-Cad, we always put the patient tissue on
a slide all by itself.  This doesn't completely solve the problem, but
it's better.
Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kuhnla,
Melissa
Sent: Wednesday, May 18, 2011 7:24 AM
To: Walter Benton; Dessoye, Michael J; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Ventana E-cadherin problem

We also experienced tissue adhesion problems. We have done several
things to address the issue:
We wear gloves when cutting controls to decrease any grease from our
fingers, we also dip the slide into the water bath upside down as to
leave the portion of the slide for the patient section untouched or
un-dipped.

Whenever repeating a slide because the first attempt fell off...we
always mount the section on a slide all by itself (no control).

We have switched to superfrost excel adhesion slides from fisher.
(we have also experienced a bad lot of slides):(

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Walter
Benton
Sent: Wednesday, May 18, 2011 10:05 AM
To: Dessoye, Michael J; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Ventana E-cadherin problem

Sounds to me like you may want to check your slide lots to make sure the
charge is consistent. I have purchased slides were there was variability
within the back from slide to slide. Also, if you use CC1 and CC2 I
would check to make sure that the solutions were not inadvertently
switched, since harsher solutions tend to wreak havoc on samples. You
may want to pH them as well to make sure they have broken down. Tech
support will give you the pH range for each solution if you don't
already know them.

I hope one of these will resolve your issue.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton <@t> cua.md
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dessoye,
Michael J [mjdessoye <@t> wvhcs.org]
Sent: Wednesday, May 18, 2011 10:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana E-cadherin problem

Hello Histonet,

We've been having a recurring problem with E-cadherin from Ventana on
Benchmark Ultra.  It's spanned two different lots of antibody.  Our
tissues are suddenly falling off the slides.  Negative controls are ok,
just seems to be after antibody treatment.  The protocol hasn't changed,
it's the manufacturer recommended protocol.  It also seems to happen to
various tissue types and fixation times.  We've tried drying the slides
longer and it doesn't seem to help.  Anyone else having problems with
this antibody?

Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health
Care System | mjdessoye <@t> wvhcs.org <mailto:mjdessoye <@t> wvhcs.org>  |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax:
570-552-1526
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