[Histonet] Freezing rodent brain

[Charles.Scouten <@t> leica-microsystems.com]

It must be completed in seconds.  Buried in powdered dry ice is one way.
Immersion in a slurry of dry ice and isopentane is another.
 
Cordially,
Charles W. Scouten, Ph.D
Product Specialist, MNL
Biosystems Division
 
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Telephone 630 964 0501
facsimile +1 630 964 0576
www.MyNeuroLab.com <http://www.myneurolab.com/> 
www.leica-microsystems.com <http://www.leica-microsystems.com/> 
 
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From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Annette
Featherstone <af46 <@t> buffalo.edu>
Sent: Tuesday, May 17, 2011 9:39 AM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Freezing rodent brain
 
How is everyone freezing hippocampus sections? The current method being
used 
seems to be causing a lot of artifact. 
Thanks 

Annette Featherstone 

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