[Histonet] Freezing rodent brain

Charles.Scouten <@t> leica-microsystems.com Charles.Scouten <@t> leica-microsystems.com
Tue May 17 09:42:10 CDT 2011

It must be completed in seconds.  Buried in powdered dry ice is one way.
Immersion in a slurry of dry ice and isopentane is another.
Charles W. Scouten, Ph.D
Product Specialist, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Telephone 630 964 0501
facsimile +1 630 964 0576
www.MyNeuroLab.com <http://www.myneurolab.com/> 
www.leica-microsystems.com <http://www.leica-microsystems.com/> 
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From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Annette
Featherstone <af46 <@t> buffalo.edu>
Sent: Tuesday, May 17, 2011 9:39 AM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Freezing rodent brain
How is everyone freezing hippocampus sections? The current method being
seems to be causing a lot of artifact. 

Annette Featherstone 

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