[Histonet] sections falling off slides

[Robin Dean]

Hi All,

 

We are trying to do IHC for multiple antigens on goat knees. The sections
are decalcified FFPE sections and we are using a Dako autostainer. We are
currently trying air-drying for  1-2 days  and baking of tissue slides
before use in IHC..

The one previous time we tried baking, we noticed a decrease in IHC signal.
The sections are pretty large, we tried cutting them down, but can't
decrease the size much.  We also  decreased the number of washes and size of
washes on the Daok, which helped only slightly.  Does anyone have any
suggestions?

 

Thank you in advance.

 

Robin

 

Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_dean <@t> compbio.com