[Histonet] Re: Histonet Digest, Vol 89, Issue 34
Mary Helie
mary.helie <@t> yale.edu
Thu May 12 13:48:22 CDT 2011
We did not plan well so getting the EBER up and running was priority
one. I just entered the 4 ml. Working up the protocol --what was best in
our hands was protease 2- 4 mins, probe, then 2 -8 min stringency washes
at 37 degrees. Perhaps now I can work on diluting it. If you get some
info on that, I would appreciate it. Thank you
On 4/28/11 1:01 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Re: Diff-Quik stain (Robert Richmond)
> 2. chicken immunohistochemistry (mohamed abd el razik)
> 3. Re: MSH 2011 Symposium (David Kemler)
> 4. IMEB (Sheila Haas)
> 5. RE: IMEB (Kaye Ryan)
> 6. RE: IMEB (sgoebel <@t> mirnarx.com)
> 7. Re: IMEB (Sheila Haas)
> 8. RE: IMEB (Bartlett, Jeanine (CDC/OID/NCEZID))
> 9. RE: IMEB (Rene J Buesa)
> 10. Ventana Probes (Angela Bitting)
> 11. Re: Ventana Probes (Angela Bitting)
> 12. Re: Ventana Probes (Rene J Buesa)
> 13. Re: Ventana Probes (Rene J Buesa)
> 14. TNF alpha antibody (Michele Wich)
> 15. RE: TNF alpha antibody (Liz Chlipala)
> 16. TNF-alpha (Liz Chlipala)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 27 Apr 2011 21:47:10 -0400
> From: Robert Richmond<rsrichmond <@t> gmail.com>
> Subject: [Histonet] Re: Diff-Quik stain
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:<BANLkTinSkBO2wNjMjEFiuRsVU=S8QB_pJA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Diff-Quik is a trade name - belongs to whatever Scientific Products is
> called this week. It's often used generically for all two-stain
> Romanowsky type stains, since there isn't a really satisfactory
> generic name for them.
>
> I've used several generic stains with separate eosin ("xanthine") and
> blue ("thiazine") dye baths, and generally found them satisfactory.
> But you do need to try them out for yourself.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 28 Apr 2011 01:10:06 -0700 (PDT)
> From: mohamed abd el razik<k84as <@t> yahoo.com>
> Subject: [Histonet] chicken immunohistochemistry
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID:<496385.41591.qm <@t> web112602.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> dear all
> i'm looking for vendors or companies that we could order some anti chicken antibodies (primary& secondary). speacially for CD4& CD8
> i'm new in that feild and need your help.
>
> mohamed
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 28 Apr 2011 03:12:07 -0700 (PDT)
> From: David Kemler<histotalk <@t> yahoo.com>
> Subject: Re: [Histonet] MSH 2011 Symposium
> To: Fellow HistoNetters<Histonet <@t> Lists.UTSouthwestern.edu>
> Message-ID:<177783.69813.qm <@t> web120605.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi Amber -
>
> I have the MSH Symposium scheduled to begin promos on May 8th's HistoTALK.
> Hopefully, the announcements will help get listeners attention and get them to
> attend. I'll be sure to mention your email on the show. :- )
>
> Yours,
> Dave
> www.HistoTALK.com
>
>
>
>
> ________________________________
> From: Amber McKenzie<amber.mckenzie <@t> gastrodocs.net>
> Cc: Histonet list serv.<histonet <@t> lists.utsouthwestern.edu>
> Sent: Wed, April 27, 2011 12:08:14 PM
> Subject: [Histonet] MSH 2011 Symposium
>
>
>
>
> If anyone is interested in attending the annual Mississippi Society for
> Histotechnology Symposium June 24-26th in Hattiesburg, MS, please
> contact me directly and I can email you a program! We'd love to have
> you :)
>
> Amber McKenzie, BS, HT(ASCP)
> MSH Secretary
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 28 Apr 2011 04:17:14 -0700 (PDT)
> From: Sheila Haas<micropathlabs <@t> yahoo.com>
> Subject: [Histonet] IMEB
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:<967305.23545.qm <@t> web161712.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Thanks to everyone for the feedback on IMEB. It appears there have only been a
> couple of successful recent transactions with them. Most of the feedback I
> received was not favorable so it appears the company is in
> a good bit of disarray for reasons unknown. At this point, I've not gotten
> a response from the owner regarding the return of our entire deposit but if
> he actually comes through, I will let you all know. In the mean time, be leary
> of future transactions with them. Thanks again for the feedback.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
>
> ------------------------------
>
> Message: 5
> Date: Thu, 28 Apr 2011 10:32:22 -0400
> From: "Kaye Ryan"<kryan <@t> nfderm.com>
> Subject: RE: [Histonet] IMEB
> To: "Sheila Haas"<micropathlabs <@t> yahoo.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DCB07C3EF84D92439E5A453D8D5B5EB066AE7E <@t> exchserver.NFDA.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible tha!
> t people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet.
>
> Kaye Ryan
> Histology Manager
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Thursday, April 28, 2011 7:17 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IMEB
>
> Thanks to everyone for the feedback on IMEB. It appears there have only been a
> couple of successful recent transactions with them. Most of the feedback I
> received was not favorable so it appears the company is in
> a good bit of disarray for reasons unknown. At this point, I've not gotten
> a response from the owner regarding the return of our entire deposit but if
> he actually comes through, I will let you all know. In the mean time, be leary
> of future transactions with them. Thanks again for the feedback.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 28 Apr 2011 09:46:09 -0500
> From:<sgoebel <@t> mirnarx.com>
> Subject: RE: [Histonet] IMEB
> To:<kryan <@t> nfderm.com>,<micropathlabs <@t> yahoo.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D957F2A7D21959488C492A2680F9920A224058 <@t> svrexch.asuragen.us>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the !
> bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!!
>
> I will now step down off my box =) Have a Happy Thursday Histo-hotties!!
>
> Sarah Goebel, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kaye Ryan
> Sent: Thursday, April 28, 2011 9:32 AM
> To: Sheila Haas; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] IMEB
>
> I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible tha!
> t people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet.
>
> Kaye Ryan
> Histology Manager
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Thursday, April 28, 2011 7:17 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IMEB
>
> Thanks to everyone for the feedback on IMEB. It appears there have only been a
> couple of successful recent transactions with them. Most of the feedback I
> received was not favorable so it appears the company is in
> a good bit of disarray for reasons unknown. At this point, I've not gotten
> a response from the owner regarding the return of our entire deposit but if
> he actually comes through, I will let you all know. In the mean time, be leary
> of future transactions with them. Thanks again for the feedback.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 28 Apr 2011 07:48:00 -0700 (PDT)
> From: Sheila Haas<micropathlabs <@t> yahoo.com>
> Subject: Re: [Histonet] IMEB
> To: Kaye Ryan<kryan <@t> nfderm.com>, histonet <@t> lists.utsouthwestern.edu
> Message-ID:<829976.69833.qm <@t> web161717.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Kaye,
> I was telling of my situation and asked if others had recent problems. I
> received many responses with two being positive and most of the others negative
> (a couple were offering suggestions on how to handle). I have heard this company
> was very reputable which is why I chose to do business with them in the first
> place but have since heard that recently things have been quite rocky. I do
> feel, at this point, that others should be leary before ordering from this
> company. I'd hate for someone else to be in the same situation as we are,
> particularly during these difficult economic times (we are out a large sum of
> money at this point). I also stated that if the issue is resolved, I would let
> everyone know but up until now it has not been.
>
> You are right, we did not have an account with IMEB but what was promised in the
> beginning is not what played out. I have been in this field for a very long time
> and realize how the accounting works but I also realize that a full refund is
> not unreasonable given I jumped through every hoop they requested I jump
> through. Keep in mind, restocking fees typically occur when items are already
> shipped or delivered to the customer and they return them. This is not the case
> here, they have both the item and our money. Again, if this issue is resolved to
> my satisfaction, I will gladly let everyone know.
>
>
>
> Sheila Haas
>
>
>
>
>
>
> ________________________________
> From: Kaye Ryan<kryan <@t> nfderm.com>
> To: Sheila Haas<micropathlabs <@t> yahoo.com>; histonet <@t> lists.utsouthwestern.edu
> Sent: Thu, April 28, 2011 10:32:22 AM
> Subject: RE: [Histonet] IMEB
>
> I usually do not respond to postings because I have seen people that may not
> agree with a posting get "roasted" by others but I felt like I had to chance it
> with this posting. I have had many excellent transactions with IMEB and would
> recommend them to anyone. Their products are trustworthy and their prices are
> excellent compared to other companies that sell the same products. I have known
> the owner and several of the reps for many years and have never heard of an
> incident like this. I too, had to put a deposit down when I made my first order
> because we did not have an established account with them. I am assuming that
> you did not have an account already established since they were checking your
> credit status? It does take time to fill out the paper work and for them to
> check your credit references. It is that way with almost any new company that
> you place a large order with. In this day and time "trust" is a hard thing to
> come by on a new account. It is terrible that people that may not have used
> them now have a negative thought in their minds about the company. We all have
> at some time something that we can find not quite to our liking about any
> company and I think we need to be careful when we are saying statements like "
> It appears there have only been a couple of successful recent transactions with
> them". This can be interpreted by some to mean that there have been many
> negative interactions so stay clear of them. Maybe a lot of their customers do
> not reply to posting or do not even subscribe to the histonet.
>
> Kaye Ryan
> Histology Manager
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Thursday, April 28, 2011 7:17 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IMEB
>
> Thanks to everyone for the feedback on IMEB. It appears there have only been a
> couple of successful recent transactions with them. Most of the feedback I
> received was not favorable so it appears the company is in
> a good bit of disarray for reasons unknown. At this point, I've not gotten
> a response from the owner regarding the return of our entire deposit but if
> he actually comes through, I will let you all know. In the mean time, be leary
> of future transactions with them. Thanks again for the feedback.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 8
> Date: Thu, 28 Apr 2011 14:48:01 +0000
> From: "Bartlett, Jeanine (CDC/OID/NCEZID)"<jqb7 <@t> cdc.gov>
> Subject: RE: [Histonet] IMEB
> To: "sgoebel <@t> mirnarx.com"<sgoebel <@t> mirnarx.com>, "kryan <@t> nfderm.com"
> <kryan <@t> nfderm.com>, "micropathlabs <@t> yahoo.com"
> <micropathlabs <@t> yahoo.com>, "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DF1CBA3D83D9A344A7D6A045188E448410FF2216 <@t> EMBX-CHAM2.cdc.gov>
> Content-Type: text/plain; charset="iso-8859-1"
>
> It would clear things up quite a bit if they would simply return her call.
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com
> Sent: Thursday, April 28, 2011 10:46 AM
> To: kryan <@t> nfderm.com; micropathlabs <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] IMEB
>
> I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the !
> bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!!
>
> I will now step down off my box =) Have a Happy Thursday Histo-hotties!!
>
> Sarah Goebel, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kaye Ryan
> Sent: Thursday, April 28, 2011 9:32 AM
> To: Sheila Haas; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] IMEB
>
> I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible tha!
> t people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet.
>
> Kaye Ryan
> Histology Manager
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Thursday, April 28, 2011 7:17 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IMEB
>
> Thanks to everyone for the feedback on IMEB. It appears there have only been a
> couple of successful recent transactions with them. Most of the feedback I
> received was not favorable so it appears the company is in
> a good bit of disarray for reasons unknown. At this point, I've not gotten
> a response from the owner regarding the return of our entire deposit but if
> he actually comes through, I will let you all know. In the mean time, be leary
> of future transactions with them. Thanks again for the feedback.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 28 Apr 2011 07:51:39 -0700 (PDT)
> From: Rene J Buesa<rjbuesa <@t> yahoo.com>
> Subject: RE: [Histonet] IMEB
> To: Sheila Haas<micropathlabs <@t> yahoo.com>,
> histonet <@t> lists.utsouthwestern.edu, Kaye Ryan<kryan <@t> nfderm.com>
> Message-ID:<119316.93664.qm <@t> web65711.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Kaye:
> In no way this posting is aimed at "roasting" yours, not at all, it is just to explain MY philosophy when participating in HistoNet.
> When I read a posting first I consider if I have something useful to contribute and if that is the case I post my answer and I really do not care if it will follow a "consensus", it is my answer based on my experience and from the start I consider that I may be wrong or that others' answers will reflect reality in a better way.
> The fact that you may have a good commercial experience with IMEB in absolutely no way excludes the possibility that others' experiences could have been disastrous.
> Additionally, it does not really matter how others have related to this company if the one posting the initial query has had a bad experience.
> Summing up do as I do: post your answer if you consider will help, and absolutely disregard what others may think of it, and never try to impose your criterion.
> René J.
>
> --- On Thu, 4/28/11, Kaye Ryan<kryan <@t> nfderm.com> wrote:
>
>
> From: Kaye Ryan<kryan <@t> nfderm.com>
> Subject: RE: [Histonet] IMEB
> To: "Sheila Haas"<micropathlabs <@t> yahoo.com>, histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, April 28, 2011, 10:32 AM
>
>
> I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new
> account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet.
>
> Kaye Ryan
> Histology Manager
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Thursday, April 28, 2011 7:17 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IMEB
>
> Thanks to everyone for the feedback on IMEB. It appears there have only been a
> couple of successful recent transactions with them. Most of the feedback I
> received was not favorable so it appears the company is in
> a good bit of disarray for reasons unknown. At this point, I've not gotten
> a response from the owner regarding the return of our entire deposit but if
> he actually comes through, I will let you all know. In the mean time, be leary
> of future transactions with them. Thanks again for the feedback.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 10
> Date: Thu, 28 Apr 2011 11:03:43 -0400
> From: "Angela Bitting"<akbitting <@t> geisinger.edu>
> Subject: [Histonet] Ventana Probes
> To: "histonet"<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:<4DB9498F.2B7F.00C9.1 <@t> geisinger.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Ok. Ventana is on my last nerve with this new probe configuration of theirs.
> I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests.
> So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka.
>
> If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me?
> Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
> -------------- next part --------------
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Bitting, Angela
> TEL;WORK:570-271-6844
> ORG:;Histology
> EMAIL;WORK;PREF;NGW:AKBITTING <@t> geisinger.edu
> N:Bitting;Angela
> END:VCARD
>
>
> ------------------------------
>
> Message: 11
> Date: Thu, 28 Apr 2011 11:10:45 -0400
> From: "Angela Bitting"<akbitting <@t> geisinger.edu>
> Subject: Re: [Histonet] Ventana Probes
> To: "Angela Bitting"<AKBITTING <@t> geisinger.edu>, "histonet"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:<4DB94B35.2B7F.00C9.1 <@t> geisinger.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts?
>
>
>>>> "Angela Bitting"<akbitting <@t> geisinger.edu> 4/28/2011 11:03 AM>>>
>>>>
> Ok. Ventana is on my last nerve with this new probe configuration of theirs.
> I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests.
> So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka.
>
> If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me?
> Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
>
>
> ------------------------------
>
> Message: 12
> Date: Thu, 28 Apr 2011 08:13:18 -0700 (PDT)
> From: Rene J Buesa<rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Ventana Probes
> To: histonet<histonet <@t> lists.utsouthwestern.edu>, Angela Bitting
> <akbitting <@t> geisinger.edu>
> Message-ID:<433054.26360.qm <@t> web65712.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Before adding vodka, take some "sips".
> René J.
>
> --- On Thu, 4/28/11, Angela Bitting<akbitting <@t> geisinger.edu> wrote:
>
>
> From: Angela Bitting<akbitting <@t> geisinger.edu>
> Subject: [Histonet] Ventana Probes
> To: "histonet"<histonet <@t> lists.utsouthwestern.edu>
> Date: Thursday, April 28, 2011, 11:03 AM
>
>
> Ok. Ventana is on my last nerve with this new probe configuration of theirs.
> I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests.
> So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka.
>
> If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me?
> Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
> -----Inline Attachment Follows-----
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 13
> Date: Thu, 28 Apr 2011 08:17:16 -0700 (PDT)
> From: Rene J Buesa<rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Ventana Probes
> To: Angela Bitting<AKBITTING <@t> geisinger.edu>, histonet
> <histonet <@t> lists.utsouthwestern.edu>, Angela Bitting
> <akbitting <@t> geisinger.edu>
> Message-ID:<533509.43351.qm <@t> web65712.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> At 100µL per dispense, to do 50 tests you will need
> 50 x 100µL = 5000 µL = 5 ml
> You do need to dilute it
> René J.
>
> --- On Thu, 4/28/11, Angela Bitting<akbitting <@t> geisinger.edu> wrote:
>
>
> From: Angela Bitting<akbitting <@t> geisinger.edu>
> Subject: Re: [Histonet] Ventana Probes
> To: "Angela Bitting"<AKBITTING <@t> geisinger.edu>, "histonet"<histonet <@t> lists.utsouthwestern.edu>
> Date: Thursday, April 28, 2011, 11:10 AM
>
>
> Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts?
>
>
>>>> "Angela Bitting"<akbitting <@t> geisinger.edu> 4/28/2011 11:03 AM>>>
>>>>
> Ok. Ventana is on my last nerve with this new probe configuration of theirs.
> I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests.
> So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka.
>
> If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me?
> Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 14
> Date: Thu, 28 Apr 2011 11:07:42 -0500
> From: "Michele Wich"<mwich <@t> 7thwavelabs.com>
> Subject: [Histonet] TNF alpha antibody
> To:<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <62A8156F8071C8439080D626DF8C33A60174E398 <@t> wave-mail.7thwave.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue?
>
> Any suggestions are greatly appreciated!
>
>
>
> ------------------------------
>
> Message: 15
> Date: Thu, 28 Apr 2011 10:15:48 -0600
> From: "Liz Chlipala"<liz <@t> premierlab.com>
> Subject: RE: [Histonet] TNF alpha antibody
> To: "Michele Wich"<mwich <@t> 7thwavelabs.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <EE33BE5C905A3046A7FF8F58A64C8E4B1B376E <@t> server.PremierLab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> We have used the antibody from abcam on FFPE mouse tissue with good
> success, have not tired it on frozen sections
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949
> fax (303) 682-9060
> www.premierlab.com
>
>
> Ship to Address:
> 1567 Skyway Drive, Unit E
> Longmont, Colorado 80504
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michele
> Wich
> Sent: Thursday, April 28, 2011 10:08 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] TNF alpha antibody
>
> Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue?
>
> Any suggestions are greatly appreciated!
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 16
> Date: Thu, 28 Apr 2011 10:30:28 -0600
> From: "Liz Chlipala"<liz <@t> premierlab.com>
> Subject: [Histonet] TNF-alpha
> To:<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <EE33BE5C905A3046A7FF8F58A64C8E4B1B3770 <@t> server.PremierLab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> I was incorrect in my earlier post, we actually use the antibody from
> Sigma for TNF- alpha, I was thinking of IL-6 that's the antibody we get
> from abcam
>
>
>
> Liz
>
>
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>
> Manager
>
> Premier Laboratory, LLC
>
> PO Box 18592
>
> Boulder, Colorado 80308
>
> office (303) 682-3949
>
> fax (303) 682-9060
>
> www.premierlab.com
>
>
>
>
>
> Ship to Address:
>
> 1567 Skyway Drive, Unit E
>
> Longmont, Colorado 80504
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 89, Issue 34
> ****************************************
>
>
>
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