[Histonet] Re: Histonet Digest, Vol 89, Issue 15
Poupak Farahani
poupakfar <@t> yahoo.com
Sun May 1 13:02:56 CDT 2011
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Sent from my iPad
On Apr 18, 2011, at 7:43 AM, histonet-request <@t> lists.utsouthwestern.edu wrote:
Woeful
LNG
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> Today's Topics:
>
> 1. Tissue transfer (Bartlett, Jeanine (CDC/OID/NCEZID))
> 2. General Data Cassette Labeler (Robin Negron)
> 3. RE: How long should autopsy/necropsy tissue be in fixation
> for a suspected TB case? (Thurby, Christina)
> 4. Re: Tissue transfer (Diane.Craft <@t> amcny.org)
> 5. Re: RE: How long should autopsy/necropsy tissue be in
> fixation for a suspected TB case? (Jay Lundgren)
> 6. Re: slide brite and coverslip drying time (Rene J Buesa)
> 7. Re: RE: How long should autopsy/necropsy tissue be in
> fixation for a suspected TB case? (Rene J Buesa)
> 8. DAB staining for Liver frozen sections (Nasreen Bashir)
> 9. RE: RE: How long should autopsy/necropsy tissue be in
> fixation for a suspected TB case? (Kumar, Devender)
> 10. Re: Tissue transfer (Rene J Buesa)
> 11. Part-time Histotech Opening (Norm Burnham)
> 12. Re: Update regarding question on Plastic Embedding (Joseph Saby)
> 13. Seeking techs all over the place! (Cheryl)
> 14. Histology Supervisor ( Hospital Scientist)-F/T (Fawaz Zouabi)
> 15. RE: RE: How long should autopsy/necropsy tissue be in
> fixation for a suspected TB case? (Tony Henwood)
> 16. RE: Tissue transfer (Kuhnla, Melissa)
> 17. Cassette labeling (Giracello, Darlene)
> 18. AKT anyone? (Amos Brooks)
> 19. [Help] oil red O stain in fattty change liver (kyoung LEE)
> 20. RE: [Help] oil red O stain in fattty change liver (Setlak, Lisa)
> 21. need monkey control tissue (Jan Shivers)
> 22. Activated Carbon (Travis Troyer)
> 23. Re: [Help] oil red O stain in fatty change liver
> (Jennifer MacDonald)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 13 Apr 2011 17:06:59 +0000
> From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
> Subject: [Histonet] Tissue transfer
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DF1CBA3D83D9A344A7D6A045188E44840E1D7758 <@t> EMBX-CHAM2.cdc.gov>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello all,
>
> We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions?
>
> Thanks!
>
> Jeanine Bartlett, BS, HT(ASCP)QIHC
> Centers for Disease Control and Prevention
> Infectious Diseases Pathology Branch
> 1600 Clifton Road, MS/G-32
> 18/SB-114
> Atlanta, GA 30333
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 13 Apr 2011 13:32:57 -0400
> From: "Robin Negron" <rnegron <@t> sarapath.com>
> Subject: [Histonet] General Data Cassette Labeler
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BC2AB06121AF1740B6417EDA9DA10C02018F0B63 <@t> MAIL.sarapath.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
> Can anyone that uses the General Data cassette labeler
> give me information regarding your overall opinion of this instrument.
>
>
>
>
>
> 1. I would like to have any information regarding the speed of
> machine. When we did a demo of this equipment it seems fast.
>
> 2. I was also questioning the cassettes, we have found that the
> only company to purchase these from is general data (at a high cost).
>
> 3. Has anyone had the equipment break down and how is the
> dependability and the customer support service.
>
> 4. Our company is very interested in the on demand version. How
> has anyone found this to be an advantage.
>
> 5. Does anyone purchase the cassettes from another dealer besides
> General Data
>
> 6. Has anyone had problems with the black scatching off.
>
> 7. We currently use TBS and they are very slow and constantly
> break down this does not meet our demand as we currently print approx.
> 400-500 per day.
>
>
>
>
>
> I would like to hear your opinions.
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 13 Apr 2011 14:04:56 -0400
> From: "Thurby, Christina" <christina.thurby <@t> bms.com>
> Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in
> fixation for a suspected TB case?
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <E9C77491626EBF41BD449EA1098B04060532A670C9 <@t> ushpwbmsmmp007.one.ads.bms.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
> Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days.
> Thanks,
> Christina Thurby
> Bristol-Myers Squibb Company
> Research Scientist I
> 812-307-2093 (tie 625)
>
>
>
>
> This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited.
>
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 13 Apr 2011 14:11:38 -0400
> From: Diane.Craft <@t> amcny.org
> Subject: Re: [Histonet] Tissue transfer
> To: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF5B30B54C.5448E72F-ON85257871.005F3643-85257871.0063F19E <@t> amcny.org>
> Content-Type: text/plain; charset="US-ASCII"
>
> I use Harleco Krystalon liquid coverslip medium. Cover the tissue, laying
> horizontally in oven for a few hours until it hardens, than soak in water
> bath, peel, and transfer to charged slide with same side facing down, dry
> in oven again, dissolve in xylene when ready for staining.
>
> Diane Craft
> Pathology Department
> Animal Medical Center
> 510 East 62nd St
> New York NY 10065-8314
> 212-329-8675 (phone)
> 212-759-5878 (fax)
>
>
>
> "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 04/13/2011 01:06 PM
>
> To
> "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> cc
>
> Subject
> [Histonet] Tissue transfer
>
>
>
>
>
> Hello all,
>
> We have some unstained slides we received from outside the U.S. on
> non-charged/un-coated slides. We would like to transfer these sections to
> charged slides before proceeding with testing. Any suggestions?
>
> Thanks!
>
> Jeanine Bartlett, BS, HT(ASCP)QIHC
> Centers for Disease Control and Prevention
> Infectious Diseases Pathology Branch
> 1600 Clifton Road, MS/G-32
> 18/SB-114
> Atlanta, GA 30333
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 13 Apr 2011 14:31:09 -0500
> From: Jay Lundgren <jaylundgren <@t> gmail.com>
> Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be
> in fixation for a suspected TB case?
> To: "Thurby, Christina" <christina.thurby <@t> bms.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BANLkTi=sP8E0fPEcuWqRcDON=-vBbdO2ng <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> As long as possible. I remember reading somewhere about TB in lung
> tissue that was still viable after *years* of 10% NBF. Make sure the lungs
> are thoroughly perfused, or they will still be unfixed in the middle. There
> is a technique to "inflate" lungs at autopsy using a large syringe full of
> NBF and a clamp. Make sure that the specimen is not contacting the walls or
> bottom of the specimen container. You can suspend the whole organ with
> string inside the container, if needed. One can also use gauze or paper
> towels to keep the organ under the surface of the fixative. Lung tissue is
> notoriously difficult to fix properly, due both to the high lipid content of
> the pleural parenchyma, and the fact that fresh lungs float.
>
> Sincerely,
>
> Jay A.
> Lundgren, M.S., HTL (ASCP)
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 13 Apr 2011 12:51:07 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] slide brite and coverslip drying time
> To: Histonet <@t> lists.utsouthwestern.edu, Dawn Herron
> <dwenzel01 <@t> gmail.com>
> Message-ID: <237269.18418.qm <@t> web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> That is exactly one of the problems with Slide Brite, and is the "trade-off" you have to pay.
> The alternative (although it may seem "odd") is to get away with alcohols and xylene altogether.
> After staining the slides, wash them in distilled water, shake them well and place them in an over at 60ºC for 5 minutes. After drying apply the mounting medium more diluted (consistency of light mineral oil), and coverslip.
> You will get away with alcohols, and xylene (or its substitutes).
> Do not "frown", just try it!
> René J.
>
> --- On Wed, 4/13/11, Dawn Herron <dwenzel01 <@t> gmail.com> wrote:
>
>
> From: Dawn Herron <dwenzel01 <@t> gmail.com>
> Subject: [Histonet] slide brite and coverslip drying time
> To: Histonet <@t> lists.utsouthwestern.edu
> Date: Wednesday, April 13, 2011, 12:16 PM
>
>
> Hello all. We have just made the transition from xylene in our linear
> stainer to Slide Brite. We are also coverslipping from Slide Brite with
> Permount. So far the slides look good (though we have to be careful about
> water contamination in the dehydrating alcohols) but we are having a problem
> with extremely long coverslipping drying time. Some of the slides have been
> on the warmer for over 18 hours and you can still move the coverslips!
> (Normally the slides would dry within 4 hours on the warmer with xylene and
> acrymount) Any suggestions on how to speed drying time?
>
> Thanks,
> Dawn
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 13 Apr 2011 12:57:28 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be
> in fixation for a suspected TB case?
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, ChristinaThurby
> <christina.thurby <@t> bms.com>
> Message-ID: <632902.43444.qm <@t> web65712.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> 5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions.
> René J.
>
> --- On Wed, 4/13/11, Thurby, Christina <christina.thurby <@t> bms.com> wrote:
>
>
> From: Thurby, Christina <christina.thurby <@t> bms.com>
> Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, April 13, 2011, 2:04 PM
>
>
> Hi,
> Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days.
> Thanks,
> Christina Thurby
> Bristol-Myers Squibb Company
> Research Scientist I
> 812-307-2093 (tie 625)
>
>
>
>
> This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 13 Apr 2011 16:07:43 -0400
> From: Nasreen Bashir <bnasreena <@t> gmail.com>
> Subject: [Histonet] DAB staining for Liver frozen sections
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BANLkTimoqrUQq4H8=ahnQGaoxOcDbES7TQ <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
> I need some information on how to block endogenous peroxidase effectively
> in liver frozen sections, as I need to do DAB staining.
> What blocking buffer will work best for blocking in liver frozen
> sections.
> I appreciate any information on this issue.
>
> Thanks,
> Reena
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 13 Apr 2011 16:08:33 -0400
> From: "Kumar, Devender" <KumarD <@t> mail.amc.edu>
> Subject: RE: [Histonet] RE: How long should autopsy/necropsy tissue be
> in fixation for a suspected TB case?
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, ChristinaThurby
> <christina.thurby <@t> bms.com>
> Message-ID:
> <DE2664B6178D5E4E9C90FF9ABE7052DA2600720C8D <@t> MS-EXCH-CCR01.AMCNT.AMC.EDU>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi,
>
> We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7 days in ABSL-3. We also confirm tissue sterility by plating the tissue homogenates on suitable media before taking the samples out of ABSL-3 facility for sectioning. In your case 7 days should be enough, but it is always better to confirm the sterility of tissue.
>
> Thanks,
> Devender
>
>
> Devender Kumar, D.V.M., Ph.D.
> Postdoctorate fellow,
> Center for Immunology & Microbial Disease,
> Albany Medical College,
> 47 New Scotland Avenue, MC
151
> Albany, NY 12208
> 518.262.6220(LAB)
> --
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Wednesday, April 13, 2011 3:57 PM
> To: histonet <@t> lists.utsouthwestern.edu; ChristinaThurby
> Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
>
> 5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions.
> René J.
>
> --- On Wed, 4/13/11, Thurby, Christina <christina.thurby <@t> bms.com> wrote:
>
>
> From: Thurby, Christina <christina.thurby <@t> bms.com>
> Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, April 13, 2011, 2:04 PM
>
>
> Hi,
> Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days.
> Thanks,
> Christina Thurby
> Bristol-Myers Squibb Company
> Research Scientist I
> 812-307-2093 (tie 625)
>
>
>
>
> This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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> ------------------------------
>
> Message: 10
> Date: Wed, 13 Apr 2011 12:54:12 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Tissue transfer
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, " Jeanine
> \(CDC/OID/NCEZID\)Bartlett" <jqb7 <@t> cdc.gov>
> Message-ID: <899879.15372.qm <@t> web65713.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> "Mission impossible" (successfully, you can always ruin them)!
> It is better to do the staining after a prolonged oven heating and do it delicately.
> René J.
>
> --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) <jqb7 <@t> cdc.gov> wrote:
>
>
> From: Bartlett, Jeanine (CDC/OID/NCEZID) <jqb7 <@t> cdc.gov>
> Subject: [Histonet] Tissue transfer
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, April 13, 2011, 1:06 PM
>
>
> Hello all,
>
> We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions?
>
> Thanks!
>
> Jeanine Bartlett, BS, HT(ASCP)QIHC
> Centers for Disease Control and Prevention
> Infectious Diseases Pathology Branch
> 1600 Clifton Road, MS/G-32
> 18/SB-114
> Atlanta, GA 30333
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 13 Apr 2011 16:52:58 -0500
> From: "Norm Burnham" <Norm.Burnham <@t> propath.com>
> Subject: [Histonet] Part-time Histotech Opening
> To: <histonet <@t> lists.utsouthwestern.edu>
> Cc: Angie.Viancourt <@t> propath.com
> Message-ID:
> <82C7248978CB50469FD6BA68EBBEFE6705653652 <@t> exchange.propathlab.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear Histonetters,
>
>
>
> We have the following opening:
>
>
>
>
>
> HISTOTECHNOLOGIST
>
>
>
> ProPath, a high volume, pathology practice, located in Dallas, Texas, has an
> immediate opening for a part-time Histotechnologist. Responsibilities
> include embedding tissue specimens, microtomy of paraffin-embedded tissue,
> operation of automated stainer and coverslipper, equipment maintenance and
> record retention.
>
>
>
> The ideal candidate will have a high school diploma or equivalent. We
> prefer, HT, HTL (ASCP) registered or eligible.
>
>
>
> The hours are 2:00 p.m. to 6:00 p.m. Monday - Friday.
>
>
>
> For consideration send resume to:
>
> ProPath, Human Resources
>
> 1355 River Bend Drive
>
> Dallas, TX 75247
>
> FAX: 214/237-1825
>
> Job-Line: 214/237-1775
>
> Email address: jobs <@t> propath.com
>
> Website: www.propath.com <http://www.propath.com/>
>
>
>
> Don't Follow the Leader! Join the Leader!
>
>
>
> EOE
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 13 Apr 2011 15:51:41 -0700 (PDT)
> From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
> Subject: Re: [Histonet] Update regarding question on Plastic Embedding
> To: Mahesh Polavarapu <polavarapu.mahesh <@t> gmail.com>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <219317.4678.qm <@t> web114401.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Mahesh-
>
> If you have access to a sonicator, you can etch the slides by first soaking them
> in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water
> rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator.
>
> You may have to work with the staining times, but you will find that many of
> your paraffin embedding stains will work.
>
> Good luck!
>
> Joe Saby, BA HT
> NAMSA
>
>
>
> ________________________________
> From: Mahesh Polavarapu <polavarapu.mahesh <@t> gmail.com>
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tue, April 12, 2011 8:25:30 PM
> Subject: [Histonet] Update regarding question on Plastic Embedding
>
> Looking for a protocol to visualize vascularization and collagen deposition
> at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic
> system. Sections will be rather thick (~50um) b/c they are being made
> through a titanium anchor. Using MMA with a cold-curing resin, Technovit
> 9100. Thanks in advance!
>
> - Mahesh
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 13 Apr 2011 18:04:50 -0700 (PDT)
> From: Cheryl <tkngflght <@t> yahoo.com>
> Subject: [Histonet] Seeking techs all over the place!
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <547837.58901.qm <@t> web39422.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
>
> Help!
>
> We're getting busy again (the economy rebounds??) and need both permanent and temporary histotechs.
>
> Are you looking for...
> ...a new place to live?
> ...a bigger city?
> ...a smaller city?
> ...a promotion?
> ...a raise?
> ...a place to learn new things?
>
> We will actively consider...
> ...new grads (and almost grads)
> ...experienced bench techs
> ...bench techs looking for a step up
> ...experienced management
> ...retirees (we know you've still 'got it')
>
> We have a number of permanent openings and seeking to successfully fill 9 thirteen-week temp positions. We are working histotechs so the conversation is easy and usually kinda fun.
> Send a resume or ask for an application -
> tkngflght <@t> yahoo.com
> Fax/Phone: 800.756.3309
>
> ------------------------------
>
> Message: 14
> Date: Thu, 14 Apr 2011 13:44:54 +1000
> From: "Fawaz Zouabi" <Fawaz.Zouabi <@t> sswahs.nsw.gov.au>
> Subject: [Histonet] Histology Supervisor ( Hospital Scientist)-F/T
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CAD5C1457A12E24B8594875C4A75E24001A7AA <@t> sswlivma04.intra.swsahs.nsw.gov.au>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> A Histology Supervisor ( Hospital Scientist) -F/T position is currently available at The Department of Forensic Medicine (Sydney local health network / Sydney south West), GLEBE 2037 NSW AUSTRALIA.
> To enquire please check the NSW health web site on http://www.health.nsw.gov.au/jobs/index.asp
> The successful applicant will be responsible of the supervision of the DOFM histology laboratory which involves the collection and processing of forensic histological specimens.
>
> <mailto:fawaz.zouabi <@t> sswahs.nsw.gov.au>
>
>
> ------------------------------
>
> Message: 15
> Date: Thu, 14 Apr 2011 06:58:57 +0000
> From: Tony Henwood <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] RE: How long should autopsy/necropsy tissue be
> in fixation for a suspected TB case?
> To: "'Thurby, Christina'" <christina.thurby <@t> bms.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884F5E6 <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Christina,
>
> The following might be useful:
>
> Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from formalin fixed lung tissue that had previously been shown to be positive by sputum culture. They were unable to culture TB from these tissues.
>
> Gerston et al (HUM PATHOL 35:571-575.2004) in South Africa analysed 138 formalin fixed lungs with histological evidence of AFB and were able to culture TB from 12 of these cases (one of these cases had been fixed for 80 days before being tested).
>
> Gerston et al suggest that there is a risk of contracting tuberculosis from tissue that has been fixed in formalin, if aerosols or accidental inoculation should occur.
>
> Trimming and sectioning wax blocks are of concern but no studies have been done yet.
>
> Of concern to histotechnolgists are:
> 1. Tissue with Inflammation-induced Encapsulation may protect bugs from formalin.
> 2. Formalin dilutes as it penetrates tissue.
> 3. Formalin substitutes may not be germicidal.
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thurby, Christina
> Sent: Thursday, 14 April 2011 4:05 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
>
> Hi,
> Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days.
> Thanks,
> Christina Thurby
> Bristol-Myers Squibb Company
> Research Scientist I
> 812-307-2093 (tie 625)
>
>
>
>
> This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited.
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *********************************************************************************
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>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
> *********************************************************************************
>
>
>
> ------------------------------
>
> Message: 16
> Date: Thu, 14 Apr 2011 07:11:02 -0400
> From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
> Subject: RE: [Histonet] Tissue transfer
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> <histonet <@t> lists.utsouthwestern.edu>, " Jeanine
> (CDC/OID/NCEZID)Bartlett" <jqb7 <@t> cdc.gov>
> Message-ID:
> <C76F12086768614F9C2618ED9966FEE10698F429 <@t> MMDCNT0BXVS003.chsli.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello,
> We do have a successful way to transfer tissue sections. It is using Mount Quik liquid coverslip. We purchase this from Newcomber Supply. Are you doing IHC testing?
> 1. H&E sections first (so they are visible)(for IHC, retrieval will fade the stain anyways)
> 2. After the stain, run slide to Xylene
> 3. Cover entire slide with layer of Mount Quik
> 4. Bake horizontal in 60 degree oven for 1 hour
> 5. Bake vertical for one hour
> 6. Rinse slide in running warm water until the mount quik and tissue lift from the slide
> 7. Divide the tissue as need, place portions on plus slide
> 8. Bake horizontal for 1 hour
> 9. Bake vertical for one hour
> 10. Dissolve off mount quik with several 10 minute steps in Xylene. No visible residual should be left
> 11. Carry out testing
>
> Hope this helps
>
> Melissa
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Wednesday, April 13, 2011 3:54 PM
> To: histonet <@t> lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett
> Subject: Re: [Histonet] Tissue transfer
>
> "Mission impossible" (successfully, you can always ruin them)!
> It is better to do the staining after a prolonged oven heating and do it delicately.
> René J.
>
> --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) <jqb7 <@t> cdc.gov> wrote:
>
>
> From: Bartlett, Jeanine (CDC/OID/NCEZID) <jqb7 <@t> cdc.gov>
> Subject: [Histonet] Tissue transfer
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, April 13, 2011, 1:06 PM
>
>
> Hello all,
>
> We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions?
>
> Thanks!
>
> Jeanine Bartlett, BS, HT(ASCP)QIHC
> Centers for Disease Control and Prevention
> Infectious Diseases Pathology Branch
> 1600 Clifton Road, MS/G-32
> 18/SB-114
> Atlanta, GA 30333
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you.
>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Thu, 14 Apr 2011 09:19:00 -0500
> From: "Giracello, Darlene" <darlene.giracello <@t> okstate.edu>
> Subject: [Histonet] Cassette labeling
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <0C2BDF59DC80E64AB2A59939D703B2E5016BD5C2EEC1 <@t> STWEXE3.ad.okstate.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Our cassette printer finally gave up on us, and we are looking at available options.
>
> Does anyone have experience with the Brady cassette labeling system? We would really appreciate ANY feedback about this product. We are also open to suggestions. We are a veterinary lab with budget constraints :-)
>
> Thank you in advance for your help
> Darlene
>
>
> ------------------------------
>
> Message: 18
> Date: Thu, 14 Apr 2011 10:25:16 -0400
> From: Amos Brooks <amosbrooks <@t> gmail.com>
> Subject: [Histonet] AKT anyone?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BANLkTinjWVVcCmkDTHvowmMQ3n28gL+ggQ <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
> I am trying to get AKT working on formalin fixed paraffin embedded human
> surgical pathology tissue. I have tried two vendors (Cell Signalling and
> Epitomics) and I'm having terrible difficulty getting anything out of it. I
> was wondering if anyone else is using AKT (from anywhere) and is getting
> decent results. The PI here is getting really upset about it and I feel like
> I've done everything but stand on my head while doing the test.
>
> Thanks,
> Amos
>
>
> ------------------------------
>
> Message: 19
> Date: Thu, 14 Apr 2011 23:07:24 +0900 (KST)
> From: kyoung LEE <east78 <@t> yahoo.co.kr>
> Subject: [Histonet] [Help] oil red O stain in fattty change liver
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <147665.31524.qm <@t> web70905.mail.kr3.yahoo.com>
> Content-Type: text/plain; charset=utf-8
>
> Dear sirs.
>
> I don't know whether I send this question to you.
>
> For several weeks, I'm setting oil red O stain in fattty change liver (mouse).
>
> Could you review my protocol?
>
> 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin).
> 2. Make frozen block
> 3. Blocks were cut 5um in crystat and dried for 1~2H at RT.Â
> 4. store at -20
> 5. let it dry for 30mins at RT
> 6. immerse it in cold 10% NBF for 10mins
> 7. staining
>
> After staining, my section were fallen off and seperated.
>
> I search many protocols. Some suggest additional fixation is necessary as soon
> as cutting.
> Others suggest prefixed tissue is not necessary additional fixation.
>
> Plz send me your comments.
>
> ------------------------------
>
> Message: 20
> Date: Thu, 14 Apr 2011 10:09:41 -0500
> From: "Setlak, Lisa" <LSetlak <@t> childrensmemorial.org>
> Subject: RE: [Histonet] [Help] oil red O stain in fattty change liver
> To: 'kyoung LEE' <east78 <@t> yahoo.co.kr>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <7111DB39D045004C9CF29E79C71B28BC101D50791C <@t> CMHEXCC01MBX.childrensmemorial.org>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We do this stain on frozen sections in our lab with no formalin fixation. We stain in oil red o from American Mastertech for 30 minutes, rinse in water, counterstain in hematoxylin, coverslip with aqueous mounting media.
> Lisa
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kyoung LEE
> Sent: Thursday, April 14, 2011 9:07 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] [Help] oil red O stain in fattty change liver
>
> Dear sirs.
>
> I don't know whether I send this question to you.
>
> For several weeks, I'm setting oil red O stain in fattty change liver (mouse).
>
> Could you review my protocol?
>
> 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin).
> 2. Make frozen block
> 3. Blocks were cut 5um in crystat and dried for 1~2H at RT.
> 4. store at -20
> 5. let it dry for 30mins at RT
> 6. immerse it in cold 10% NBF for 10mins
> 7. staining
>
> After staining, my section were fallen off and seperated.
>
> I search many protocols. Some suggest additional fixation is necessary as soon
> as cutting.
> Others suggest prefixed tissue is not necessary additional fixation.
>
> Plz send me your comments.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 21
> Date: Thu, 14 Apr 2011 10:56:11 -0500
> From: "Jan Shivers" <shive003 <@t> umn.edu>
> Subject: [Histonet] need monkey control tissue
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <0B3AF0D33B2447EF910223B1D49D1226 <@t> auxs.umn.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello,
> I'm posting this request for a colleague, who is in need of normal monkey placenta (preferably Rhesus). If anyone has some to spare, please let me know and I'll forward your response.
>
> Thanks,
> Jan Shivers
> Senior Scientist
> Histology/IHC/EM Section Head
> Pathology Teaching Program
> University of Minnesota
> Veterinary Diagnostic Laboratory
> 1333 Gortner Ave.
> St. Paul, MN 55108
> 612-624-7297
> shive003 <@t> umn.edu
>
> (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.)
>
>
> ------------------------------
>
> Message: 22
> Date: Thu, 14 Apr 2011 11:08:21 -0500
> From: "Travis Troyer" <ttroyer <@t> petersonlab.com>
> Subject: [Histonet] Activated Carbon
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <367C74D495C248669E61229AFF3B60EB <@t> Peterson.local>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an inexpensive vendor for activated carbon for the processor?
>
> Thanks,
> Travis
>
> ------------------------------
>
> Message: 23
> Date: Thu, 14 Apr 2011 09:10:33 -0700
> From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> Subject: Re: [Histonet] [Help] oil red O stain in fatty change liver
> To: kyoung LEE <east78 <@t> yahoo.co.kr>
> Cc: histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFEB80E5B0.D656A61E-ON88257872.00587439-88257872.0058DBC9 <@t> mtsac.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> We use pre-fixed tissue for oil red o. We rinse the section briefly to
> remove the formalin from the outside of the tissue. We prepare our tissue
> in OCT or the equivalent. We cut the frozen sections and mount them on
> charged slides. We let dry the dry a minimum of 10 minute, rinse them in
> water to remove the OCT and then proceed with the stain.
>
>
>
>
> kyoung LEE <east78 <@t> yahoo.co.kr>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 04/14/2011 08:04 AM
>
> To
> histonet <@t> lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] [Help] oil red O stain in fattty change liver
>
>
>
>
>
>
> Dear sirs.
>
> I don't know whether I send this question to you.
>
> For several weeks, I'm setting oil red O stain in fattty change liver
> (mouse).
>
> Could you review my protocol?
>
> 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered
> formalin).
> 2. Make frozen block
> 3. Blocks were cut 5um in crystat and dried for 1~2H at RT.
> 4. store at -20
> 5. let it dry for 30mins at RT
> 6. immerse it in cold 10% NBF for 10mins
> 7. staining
>
> After staining, my section were fallen off and seperated.
>
> I search many protocols. Some suggest additional fixation is necessary as
> soon
> as cutting.
> Others suggest prefixed tissue is not necessary additional fixation.
>
> Plz send me your comments.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 89, Issue 15
> ****************************************
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