[Histonet] PAS for fonfus
shrivastavaanuradha <@t> hotmail.com
Tue Mar 29 17:57:28 CDT 2011
I am doing PAS for Fungus with SCHIFF’S and counter –staining with Hematoxylin, the Hyphe look little paler than the spores/ yeast. Our Doc. wants the hyphe to be dark too. Any suggestion.
Ikeep in Periodic acid for 5 min. nice rinse then in schiff’s for 15 min. then wash for 10 mi, in running tap water. then counterstain. Pleaseneed your expert advice. thank you.
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