[Histonet] PAS for fonfus

[anuradha shrivastava]

Hi Histonetters,
I am doing PAS for Fungus with SCHIFF’S and counter –staining with Hematoxylin, the Hyphe look little paler than the spores/ yeast. Our Doc. wants the hyphe to be dark too. Any suggestion.
Ikeep in Periodic acid for 5 min. nice rinse then in schiff’s for 15 min. then wash for 10 mi, in running tap water. then counterstain. Pleaseneed your expert advice. thank you.