[Histonet] PAS for fonfus

anuradha shrivastava shrivastavaanuradha <@t> hotmail.com
Tue Mar 29 17:57:28 CDT 2011

Hi Histonetters,
I am doing PAS for Fungus with SCHIFF’S and counter –staining with Hematoxylin, the Hyphe look little paler than the spores/ yeast. Our Doc. wants the hyphe to be dark too. Any suggestion.
Ikeep in Periodic acid for 5 min. nice rinse then in schiff’s for 15 min. then wash for 10 mi, in running tap water. then counterstain. Pleaseneed your expert advice. thank you.

More information about the Histonet mailing list