[Histonet] RE: Histonet Digest, Vol 88, Issue 33
Brenda Royce
broyce <@t> NSH.org
Fri Mar 25 13:59:22 CDT 2011
To all NSH Members - Don't forget to submit your contest entry form on how your lab celebrated HPD!
Due by March 31, 2011 for chance to win free education for the entire lab.
Visit http://www.nsh.org/content/histotechnology-day for more information.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, March 25, 2011 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 88, Issue 33
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Today's Topics:
1. GREAT B5 substitute (Akemi Allison)
2. bone marrow biopsy decals (Diana Martinez-Longoria)
3. Re: bone marrow biopsy decals (Akemi Allison)
4. RE: Stupid rabbit antibodies (Perry, Margaret)
5. Re: GREAT B5 substitute (Akemi Allison)
6. Great Pathology Laboratory Manager position in NC (Alisha Dynan)
7. part time/ per diem Job opportunity in San Diego, CA
( Susan A. Kelly )
8. Any body interested to sell the microscope ?
(amitapandey <@t> torrentpharma.com)
9. RE: bone marrow biopsy decals (Patsy Ruegg)
10. RE: RE: Microsatellite Instability Antibodies (Patsy Ruegg)
11. Vaginal Smears-Fixation (Amber Tyler)
12. (no subject) (Gaiser, Marcia)
13. RE: RE: Stupid rabbit antibodies (Patsy Ruegg)
----------------------------------------------------------------------
Message: 1
Date: Thu, 24 Mar 2011 10:07:38 -0700 (PDT)
From: Akemi Allison <akemiat3377 <@t> yahoo.com>
Subject: [Histonet] GREAT B5 substitute
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <465432.55346.qm <@t> web113805.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=utf-8
Hi All,
I used to use B5, Zenker's and Helly's for years on LN and BM biopsies. I wanted to stop using mercury in our department, but didn't want to compromise the quality of the results we were getting.
I was interested in using fixatives and decalcification products that would not effect IHC results. At the suggestion of Dr. Elaine Jaffe, Chief Hematopathologist from NIH, I tried 2 products from BBC Biochemical. I did parallel studies on bilateral BM bx's using B•Plus Fixative™ and RapidCal•Immuno™, and then B5 fixative and EDTA decal solution, and then Zenker's fixative. We then did parallel studies for IHC. We ran a panel of Ab's on specimens which were treated with each of the solutions.
The pathologists concluded that B•Plus Fixative™ produces superb fixation for hematopoietic tissues and the RapidCal•Immuno™ does not interfere with IHC staining. In some cases, the results on the IHC's were better on some of the Ab's using these products too.
It only takes 2-3 hours of fixation time for BM biopsies. After prefixing, they can go directly into RapidCal•Immuno™ for 45 minutes to 1 hour, washed in running water for 5 minutes, and then continue to be processed as normal starting off in 10% formalin. Just a note: This product is a rapid decalcifier designed to preserve immunogenicity at a maximum while accomplishing rapid decalcification. It is designed specifically for bone marrow biopsies and LN biopsies.
By the way, I am not on BBC's payroll. I am always on a quest to improve patient care!
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
------------------------------
Message: 2
Date: Thu, 24 Mar 2011 10:00:57 -0700
From: Diana Martinez-Longoria <dmlongoria <@t> ecrmc.org>
Subject: [Histonet] bone marrow biopsy decals
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D15A78C6-9808-4BB0-BED3-5F5266431D9D <@t> mimectl>
Content-Type: text/plain; charset="iso-8859-1"
Our pathologist wants to know why Formical-4 is the best decal for bone marrow biopsies, since we heard about in Histonet? I have experimented with four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid and we are still not sure which will be the best decal. Also we a decal that will minimize the leeching out of iron. If any out there has any suggest, please let me know. Thanks in advance!
Diana Martinez-Longoria
Histotechnician HT(ASCP)CM
El Centro Regional Medical Center
1415 Ross Ave
El Centro CA 92243
(760) 339-7267
(760)482-5365 fax
dmlongoria <@t> ecrmc.org
Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments.
ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
------------------------------
Message: 3
Date: Thu, 24 Mar 2011 11:17:22 -0700
From: Akemi Allison <akemiat3377 <@t> yahoo.com>
Subject: Re: [Histonet] bone marrow biopsy decals
To: Diana Martinez-Longoria <dmlongoria <@t> ecrmc.org>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <62D903C5-EF3C-4792-ABD2-962E5A72A602 <@t> yahoo.com>
Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed
Hi Diana,
I recently made a histonet posting "Great B5 substitute" which also addresses BM decal. Since there are a number of companies who sell decal solutions with similar names, I am not sure you tried the products from BBC Chemical. It is best to use both products together. My post goes into my findings.
Regards,
Akemi
Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com
On Mar 24, 2011, at 10:00 AM, Diana Martinez-Longoria wrote:
> Our pathologist wants to know why Formical-4 is the best decal for
> bone marrow biopsies, since we heard about in Histonet? I have
> experimented with four different decals: Rapid-Immuno, Formical-4,
> Nitric Acid, and HCL\Formic Acid and we are still not sure which will
> be the best decal. Also we a decal that will minimize the leeching
> out of iron. If any out there has any suggest, please let me know.
> Thanks in advance!
>
> Diana Martinez-Longoria
> Histotechnician HT(ASCP)CM
> El Centro Regional Medical Center
> 1415 Ross Ave
> El Centro CA 92243
> (760) 339-7267
> (760)482-5365 fax
>
> dmlongoria <@t> ecrmc.org
>
>
>
>
>
>
> Confidentiality Notice: This e-mail is for the sole use of the
> intended recipient(s) and may contain confidential and privileged
> information. Any unauthorized review, use, disclosure, or distribution
> is prohibited. If you are not the intended recipient, please contact
> the sender at the phone number above and promptly destroy this e-mail
> and its attachments.
>
>
> ECRMC Confidentiality Notice: This e-mail is for the sole use of the
> intended recipient(s) and may contain confidential and privileged
> information. Any unauthorized review, use, disclosure, or distribution
> is prohibited. If you are not the intended recipient, PLEASE contact
> the sender and promptly destroy this e- mail and its attachments.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 24 Mar 2011 14:05:00 -0500
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] RE: Stupid rabbit antibodies
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FCA5EF47F9BC694CBB4C58FEA042196351E7FB30AE <@t> SDSU-MBX.jacks.local>
Content-Type: text/plain; charset="us-ascii"
Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit specific HRP and used Nova red from Vector or the DAB in the kit depending on which antibody we used. It is excellent , maybe even a little better than Dako's envision. I did have to use blocks such as FC receptor block Innovex, Powerblock Biogenex and serum free protein block DAKO. What I used depended on the antibody with some needing only the block provided in the kit. Good Luck.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007
605-688-5638
------------------------------
Message: 17
Date: Tue, 22 Mar 2011 16:04:16 -0500
From: <sgoebel <@t> mirnarx.com>
Subject: [Histonet] Stupid Rabbit primaries!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A1C5FF5 <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="us-ascii"
So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal. Does
anyone have any suggestions for how to eliminate the background? I have
diluted almost to the point of the antigens not showing! Thanks guys
and gals!!
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
------------------------------
Message: 18
Date: Tue, 22 Mar 2011 16:13:04 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Stupid Rabbit primaries!
To: <sgoebel <@t> mirnarx.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8C023B4AB999614BA4791BAEB26E2738010029DE <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Do you know for sure that its the Ab and not endogenous biotin,
peroxidase or Phosphatase? You can block for all those.
Otherwise there are a number of commercial blocking agents on the
market...try Biocare for a start (800)799-9499 or better yet, the
company that makes your antibody.
Good luck.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
------------------------------
Message: 5
Date: Thu, 24 Mar 2011 13:03:40 -0700
From: Akemi Allison <akemiat3377 <@t> yahoo.com>
Subject: Re: [Histonet] GREAT B5 substitute
To: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1FB5801D-95F0-437B-A884-E98491E1769E <@t> yahoo.com>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
Having worked and developed products in the Biotech arena, not all
products are disclosed. If they are, you lose the competitive edge.
Here is the link to the the MSDS and information BCC discloses;
http://www.bbcus.com/uploads/files/BPlusFix_MSDS.pdf
Section 2. Composition and Information on Ingredients
Name CAS# % by Weight Exposure Limits
1) Water
2) Formaldehyde 37%
3) Zinc Salts
4) Selected Buffers
7732-18-5
50-00-0
Not Available
Not Available
<25%
>0.5% - <1%
Not Available
Not Available
OSHA PEL 0.75ppm TWA
Not Available
Not Available
Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com
On Mar 24, 2011, at 12:28 PM, Johnson, Nacaela wrote:
> Are the contents of the B Plus Fixative proprietary? Is it an AZF
> type
> of fixative? Did you try any ISH on the test runs?
>
>
> Thanks,
>
> Nacaela Johnson, HTL (ASCP)
> Histotechnologist
> KCCC Pathology
> 12000 110th St., Ste. 400
> Overland Park, KS 66210
> Office: 913-234-0576
> Fax: 913-433-7639
> Email: Nacaela.Johnson <@t> USOncology.com
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
> Allison
> Sent: Thursday, March 24, 2011 12:08 PM
> To: histonet
> Subject: [Histonet] GREAT B5 substitute
>
> Hi All,
> I used to use B5, Zenker's and Helly's for years on LN and BM
> biopsies.
> I wanted to stop using mercury in our department, but didn't want to
> compromise the quality of the results we were getting.
>
> I was interested in using fixatives and decalcification products that
> would not effect IHC results. At the suggestion of Dr. Elaine Jaffe,
> Chief Hematopathologist from NIH, I tried 2 products from BBC
> Biochemical. I did parallel studies on bilateral BM bx's using B*Plus
> Fixative(tm) and RapidCal*Immuno(tm), and then B5 fixative and EDTA
> decal solution, and then Zenker's fixative. We then did parallel
> studies for IHC. We ran a panel of Ab's on specimens which were
> treated
> with each of the solutions.
>
> The pathologists concluded that B*Plus Fixative(tm) produces superb
> fixation for hematopoietic tissues and the RapidCal*Immuno(tm) does
> not
> interfere with IHC staining. In some cases, the results on the IHC's
> were better on some of the Ab's using these products too.
>
> It only takes 2-3 hours of fixation time for BM biopsies. After
> prefixing, they can go directly into RapidCal*Immuno(tm) for 45
> minutes
> to 1 hour, washed in running water for 5 minutes, and then continue to
> be processed as normal starting off in 10% formalin. Just a note:
> This
> product is a rapid decalcifier designed to preserve immunogenicity
> at a
> maximum while accomplishing rapid decalcification. It is designed
> specifically for bone marrow biopsies and LN biopsies.
>
> By the way, I am not on BBC's payroll. I am always on a quest to
> improve patient care!
>
> Akemi Allison BS, HT(ASCP)HTL
> Director
> Phoenix Lab Consulting
> E-Mail: akemiat3377 <@t> yahoo.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> </pre>The contents of this electronic mail message and any
> attachments are confidential, possibly privileged and intended for
> the addressee(s) only.<br>Only the addressee(s) may read,
> disseminate, retain or otherwise use this message. If received in
> error, please immediately inform the sender and then delete this
> message without disclosing its contents to anyone.</pre>
>
------------------------------
Message: 6
Date: 24 Mar 2011 17:51:45 -0400
From: Alisha Dynan <alisha <@t> ka-recruiting.com>
Subject: [Histonet] Great Pathology Laboratory Manager position in NC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <825205274.1301003505037.JavaMail.cfservice <@t> SL4APP4>
Content-Type: text/plain; charset="utf-8"
Dear Histonet Members,
I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below are some Histotechnologist positions we are currently working on:
Highlighted Job Opportunity: Pathology Laboratory Manager in Western NC
I am currently working with a large hospital system in Western NC that is looking to hire a Pathology Laboratory Manager. This non-profit independent community hospital is the busiest surgical hospital in the state. My client is looking for an experienced histology professionals with technical expertise in all aspects of histology, IHC, coding/billing. Experience with CoPath is a plus. My client is looking for someone who is HT or HTL(ASCP) certified and has at least 2 years of management experience at a medium to large sized hospital. This area of NC has been recognized as one of the top travel destinations in the world and one of the top 10 places to live in the United States. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits.If interested in learning more, please email me at alisha <@t> ka-recruiting.com.
If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.
If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.
To view some additional opportunities please visit our website at www.ka-recruiting.com.
Sincerely,
Alisha Dynan, Founder
K.A. Recruiting, Inc.
Your Partner in Healthcare Recruiting
10 Post Office Square 8th Floor SOUTH
Boston, MA 02109
P: (617) 692-2949
F: (617) 507-8009
alisha <@t> ka-recruiting.com
www.ka-recruiting.com
------------------------------
Message: 7
Date: Thu, 24 Mar 2011 18:44:46 -0400
From: " Susan A. Kelly " <sukey <@t> compassdermpath.com>
Subject: [Histonet] part time/ per diem Job opportunity in San Diego,
CA
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<20110324184446.79wfuds5naosoc8w <@t> webmail.compassdermpath.com>
Content-Type: text/plain; charset=UTF-8
Hello, Fellow Histonetters;
I am currently in search of an ASCP registered Histotechnician to cover vacations in our new Dermatopathology laboratory. The hours are flexible and the current need for coverage will be the last 2 weeks in May. There would be an overlap of 3-4 days to orient you to the daily schedule. There is great potential for the position to work into a full time.
The lab is fully equipped with top of the line products. Daily procedures include processing, embedding, sectioning, automated H&E staining, special stains and automated Immunohistochemistry as needed. We also perform cryosectioning and one-step Immunofluorescence staining (set up to run on the automated IHC stainer).
Please contact me if you have any additional questions or are interested in the position. Our two dermatopathologist are a pleasure to work with.
I look forward to hearing from interested parties.
Kind Regards,
Susan
_____________________________________
Susan A. Kelly, H.T., H.T.L.(ASCP)QIHC
Lab Manager
Compass Dermatopathology, Inc.
7300 Girard Ave, Suite 104
LaJolla, CA 92037
Phone: (858)750-2983
Fax: (858)750-2984
sukey <@t> compassdermpath.com
------------------------------
Message: 8
Date: Fri, 25 Mar 2011 09:56:52 +0530
From: amitapandey <@t> torrentpharma.com
Subject: [Histonet] Any body interested to sell the microscope ?
To: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFB1586DB9.ED3343DE-ON6525785E.0017907A-6525785E.001853C3 <@t> torrentpharma.com>
Content-Type: text/plain; charset="US-ASCII"
Dear Histonet members,
I am out in market to buy a upright light microscope. (I am looking for
lower to medium end microscope- Nikon 50i/ Zeiss Axiolab etc.)
Any body interested to sell their old but good working microscope , please
contact me.
Additionally If it has camera attach or image analysis software, it will
be benefit to me but not the essential one.
Amita
------------------------------
Message: 9
Date: Fri, 25 Mar 2011 09:22:11 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] bone marrow biopsy decals
To: "'Diana Martinez-Longoria'" <dmlongoria <@t> ecrmc.org>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E49C95C0FB7941E085D97481A922D9A0 <@t> prueggihctechlt>
Content-Type: text/plain; charset="iso-8859-1"
Diana,
I have a lot of experience with decal reagents and Formic acid is the best
in my experience for IHC. It is not as fast as the rapid decals but is the
kindest at not adversely affecting the antigens and more gentle on iron
leaching, although all decals will leach iron, we usually do iron on the
smear or clot section. Not only does the formic acid leach the iron so does
the alcohols and solvents in tissue processing.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
Martinez-Longoria
Sent: Thursday, March 24, 2011 11:01 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bone marrow biopsy decals
Our pathologist wants to know why Formical-4 is the best decal for bone
marrow biopsies, since we heard about in Histonet? I have experimented with
four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic
Acid and we are still not sure which will be the best decal. Also we a decal
that will minimize the leeching out of iron. If any out there has any
suggest, please let me know. Thanks in advance!
Diana Martinez-Longoria
Histotechnician HT(ASCP)CM
El Centro Regional Medical Center
1415 Ross Ave
El Centro CA 92243
(760) 339-7267
(760)482-5365 fax
dmlongoria <@t> ecrmc.org
Confidentiality Notice: This e-mail is for the sole use of the intended
recipient(s) and may contain confidential and privileged information. Any
unauthorized review, use, disclosure, or distribution is prohibited. If you
are not the intended recipient, please contact the sender at the phone
number above and promptly destroy this e-mail and its attachments.
ECRMC Confidentiality Notice: This e-mail is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. Any unauthorized review, use, disclosure, or distribution is
prohibited. If you are not the intended recipient, PLEASE contact the sender
and promptly destroy this e-mail and its attachments.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Fri, 25 Mar 2011 09:25:16 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] RE: Microsatellite Instability Antibodies
To: "'Weems, Joyce'" <JWeems <@t> sjha.org>, "'Hoekert, W.E.J.'"
<W.E.J.Hoekert <@t> olvg.nl>, "'Evans, Andria B'" <aevans3 <@t> lghealth.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <40B8402295F54422B2C4638E76323F1A <@t> prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"
I use the NovaCastra MSH-2 and MLH-1 on the Leica Bond Maxx.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Thursday, March 24, 2011 9:49 AM
To: Hoekert, W.E.J.; Evans, Andria B; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Microsatellite Instability Antibodies
We use Leica Bond Max and Biocare antibodies work very well for us. We're
working on perfecting the Leica antibodies as well. J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hoekert,
W.E.J.
Sent: Thursday, March 24, 2011 11:18
To: Evans, Andria B; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microsatellite Instability Antibodies
>We are currently running the Ventana IHC stainers with the UltraView
Detection.
Just like us!
MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation.
MSH-2: Cell Marque (ref 760-4265). 60' cc1, 60' ab inc.
PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc.
+amplification
MSH6: Becton Dickinson, clone 44, cat nr 610919. 60' cc2, 60' ab inc.
MSH-6 does not give very nice results, however, it works.
Good Luck,
Willem
________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Evans, Andria B
Verzonden: do 24-3-2011 13:31
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] Microsatellite Instability Antibodies
I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2)
and need some input on the protocol that you may be running in your lab and
what clone(manufacture) you are using.
We are currently running the Ventana IHC stainers with the UltraView
Detection.
Thanks for your help!
Andria B Evans HTL(ASCP)CM
This email was sent securely from the LGHealth Email Service
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If you are not the intended recipient, please contact the sender by reply
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Message: 11
Date: Fri, 25 Mar 2011 11:06:00 -0500
From: Amber Tyler <atyler <@t> pcg.wustl.edu>
Subject: [Histonet] Vaginal Smears-Fixation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTinUPzZ4iZM=HwPENX4-dm1GApSpEwQ6No4epBbP <@t> mail.gmail.com>
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Good Morning,
I am doing a study on changes in estrous cycling in mice over the long term.
I will be collecting vaginal smears daily and staining with new methylene
blue. I need to be able to keep the slides "fresh" for viewing for
approximately a week.
Does anyone have any advice on the best manner of fixing/mounting procedures
for this?
I have little experience with this. In previous studies, the slides were
only needed briefly and discarded.
Thank you,
Amber
--
Amber N. Tyler, Ph.D.
Staff Scientist
Anatomy & Neurobiology
------------------------------
Message: 12
Date: Fri, 25 Mar 2011 11:29:21 -0500
From: "Gaiser, Marcia" <Marcia_Gaiser <@t> ssmhc.com>
Subject: [Histonet] (no subject)
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<728F817C02110E498D803A7C3B0C6248068B1F5B38 <@t> S009-APEXM06.ds.ad.ssmhc.com>
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St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT or experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com<http://www.saintsok.com/<https://mobile.ssmhc.com/owa/UrlBlockedError.aspx<https://mobile.ssmhc.com/owa/UrlBlockedError.aspx<https://mobile.ssmhc.com/owa/UrlBlockedError.aspx>>>>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105.
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Message: 13
Date: Fri, 25 Mar 2011 10:51:01 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] RE: Stupid rabbit antibodies
To: "'Perry, Margaret'" <Margaret.Perry <@t> sdstate.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <41519003ECD14D6A9E27647AD10208D0 <@t> prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"
Are you using the rab abs on human samples or another species? For most
purposes serum free protein block should be all you need. If you were using
rab ab on rabbits I would recommend the FC block from Innogenex, you can
also get a anti rab link that is Fab2 and perhaps absorbed to the species
you are working with. Southern Biotech sells really good secondary link
reagents, u can get it hrp, ap, fitc, or unlabeled and then match up the
detection to the animal the link is made in. are you using a avidin/biotin
detection system? If you are there may be endogenous biotin which can be
blocked.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry,
Margaret
Sent: Thursday, March 24, 2011 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Stupid rabbit antibodies
Have you tried the Dako Rabbit envision? We also tried the AbCam Expose
Rabbit specific HRP and used Nova red from Vector or the DAB in the kit
depending on which antibody we used. It is excellent , maybe even a little
better than Dako's envision. I did have to use blocks such as FC receptor
block Innovex, Powerblock Biogenex and serum free protein block DAKO. What
I used depended on the antibody with some needing only the block provided in
the kit. Good Luck.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
------------------------------
Message: 17
Date: Tue, 22 Mar 2011 16:04:16 -0500
From: <sgoebel <@t> mirnarx.com>
Subject: [Histonet] Stupid Rabbit primaries!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A1C5FF5 <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="us-ascii"
So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal. Does
anyone have any suggestions for how to eliminate the background? I have
diluted almost to the point of the antigens not showing! Thanks guys
and gals!!
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
------------------------------
Message: 18
Date: Tue, 22 Mar 2011 16:13:04 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Stupid Rabbit primaries!
To: <sgoebel <@t> mirnarx.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8C023B4AB999614BA4791BAEB26E2738010029DE <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Do you know for sure that its the Ab and not endogenous biotin,
peroxidase or Phosphatase? You can block for all those.
Otherwise there are a number of commercial blocking agents on the
market...try Biocare for a start (800)799-9499 or better yet, the
company that makes your antibody.
Good luck.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
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End of Histonet Digest, Vol 88, Issue 33
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