[Histonet] Stupid Rabbit primaries!
Sebree Linda A
LSebree <@t> uwhealth.org
Tue Mar 22 16:13:04 CDT 2011
Do you know for sure that its the Ab and not endogenous biotin,
peroxidase or Phosphatase? You can block for all those.
Otherwise there are a number of commercial blocking agents on the
market...try Biocare for a start (800)799-9499 or better yet, the
company that makes your antibody.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: Tuesday, March 22, 2011 4:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!
So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal. Does
anyone have any suggestions for how to eliminate the background? I have
diluted almost to the point of the antigens not showing! Thanks guys
Sarah Goebel, BA, HT(ASCP)
2150 Woodward Street
Austin, Texas 78744
(512)901-0900 ext. 6912
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