[Histonet] Stupid Rabbit primaries!

Sebree Linda A LSebree <@t> uwhealth.org
Tue Mar 22 16:13:04 CDT 2011

Do you know for sure that its the Ab and not endogenous biotin,
peroxidase or Phosphatase?  You can block for all those.

Otherwise there are a number of commercial blocking agents on the
market...try Biocare for a start (800)799-9499 or better yet, the
company that makes your antibody.

Good luck. 

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: Tuesday, March 22, 2011 4:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal.  Does
anyone have any suggestions for how to eliminate the background?  I have
diluted almost to the point of the antigens not showing!  Thanks guys
and gals!!


Sarah Goebel, BA, HT(ASCP)


Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912


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