[Histonet] how to bleach melanoma cases without bad effect on IHC

Shahram Sabeti sabeti_shahram <@t> yahoo.com
Tue Mar 22 03:40:08 CDT 2011

hello dear fellows,
   i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me .


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