[Histonet] PAS for basement membrane

[Geoff McAuliffe]

Make the periodic acid fresh that day.
Use 3 2 minute washes in 0.5% sodium meta-bisulfite instead of water.

Geoff

On 3/15/2011 10:16 AM, Martin, Erin wrote:
> Hi all,
>
> I'm having a problem with my PAS stain.  My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale.  We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water.  Any thoughts?  Thank you in advance!
>
> Thanks
> Erin
>
> Erin Martin, Histology Supervisor
> UCSF Department of Dermatopathology
> 415-353-7248
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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