[Histonet] Control Slides

[Harrison, Sandra C.]

We dry the control slide at the same time that we dry the tissue being
stained, since controls are supposed to be handled in the same manner as
the test tissue.  That being said, I guess if we were truly following
that rule, we would cut the control on the same day we cut the test
sample.  :-)


"Other people say that, after 
they dry the slide, they dip the slide in paraffin, to cover the tissue,
so 
that the air doesn't touch the tissue during the months before the slide
is 
used in a stain."

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee &
Peggy Wenk
Sent: Thursday, March 10, 2011 6:39 PM
To: Amador, Amanda; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Control Slides

I've noticed that our spirochete control slides don't stain as intense 
starting about 3 months after they are sectioned, and that they stop 
staining by about 6 months. (This is with a silver stain.)

I've talked with other histotechs, and they say they've seen the same 
phenomenon with AFB and Gram controls. (We use ours up too quickly - we 
never have 6-12 months old control slides for these, so I can't attest
to 
this. )

Bancroft's book talks about this phenomenon with amyloid, and suggests
that 
oxidation of the tissue (proteins) due to exposure to air may be the
cause. 
I'm guessing the it probably applies to the precut microorganism control

slides, too.

We only cut enough slides for 6 months, and date them. Other people say
they 
put their cut control slides in a slide box with a lid after drying, and

then place the box in the refrig, as cold slows down the chemical
change, 
and the lid keeps the moving air off the slide. Other people say that,
after 
they dry the slide, they dip the slide in paraffin, to cover the tissue,
so 
that the air doesn't touch the tissue during the months before the slide
is 
used in a stain. Just 3 suggestions to stop this problem.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

--------------------------------------------------
From: "Amador, Amanda" <aamador <@t> ameripath.com>
Sent: Wednesday, March 09, 2011 10:22 PM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Control Slides

> Is there guidelines for how long special stains controls are good for
once 
> they are cut?  We have spirochetes for our Steiner that is from 2007
and 
> we are having issues.
>
> Amanda Amador, HT(ASCP)CM
> AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite
A | 
> Indianapolis, IN  46219 | phone 317.275.8052 | 
> aamador <@t> ameripath.com<mailto:aamador <@t> ameripath.com> | 
> www.AmeriPath.com<http://www.ameripath.com/>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


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