[Histonet] immuno stain for nerve in arterial wall

[Young Kwun]

Hi Cindy,

You would need to know what kinds of nerves your investigator is interested
in. 
Sympathetic adrenergic nerves travel along arteries and nerves and are found
in the adventitia (outer wall of a blood vessel). Varicosities, which are
small enlargements along the nerve fibers, are the site of neurotransmitter
release. 
Parasympathetic fibers are also found associated with blood vessels in
certain organs such as salivary glands, gastrointestinal glands, and in
genital erectile tissues.

If interested in both fibers, you could start with antibodies
(fluorescent-conjugated) such as Thyrosine hydroxylase, dopamine, serotonin,
substance P, Neuropeitide Y, acetylcholine, VIP, CGRP etc., etc..

Cheers,

Young




Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Repatriation General Hospital
Concord NSW 2139
 
02-9767-6075 (Tel)
02-9767-8427 (Fax)
kwuny <@t> email.cs.nsw.gov.au






-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of C B
Sent: Saturday, March 05, 2011 10:06 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] immuno stain for nerve in arterial wall

We had an investigator inquire about flourescent immuno stains for nerve in 
arterial wall (not sure what species they are working with).  I've never
worked 
with nerve before.  Can someone give me some advice about what primary
should I 
be searching for?  I realize I have to look in to species cross reactivity
once 
I get that information, but I don't even know what to "search" for.
Thank you,
Cindy Baranowski
SJTRI Atlanta, GA


      
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