[Histonet] bone marrow aspirations

DKBoyd <@t> chs.net DKBoyd <@t> chs.net
Thu Mar 3 08:34:46 CST 2011

We let our smears air dry and then we stain them on the Wright stainer, in 
which the first step is a fixative.  We reserve one slide for an FE stain, 
this slide is fixed in Methanol for 10 mins, then continue per usual with 
the FE stain.
Hope this helps!

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net

Debra Siena <DSiena <@t> statlab.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/02/2011 10:49 AM

"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>

[Histonet] bone marrow aspirations

Hi All,

I would appreciate some insight as to how most labs are treating bone 
marrow smears, in regards to fixation.  I am not embarrassed to say that 
it has been awhile since I worked with them.  With the smears are most 
labs air drying, methanol (alcohol fixation) or spray fixation (pap 
fixative) or something else?  I do thank you for your help.

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. | McKinney, TX 75071
Direct: 972-436-1010  x229 | Fax: 972-436-1369
dsiena <@t> statlab.com<mailto:maubrey <@t> statlab.com> | www.statlab.com<

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