[Histonet] bone marrow aspirations

[DKBoyd <@t> chs.net]

We let our smears air dry and then we stain them on the Wright stainer, in 
which the first step is a fixative.  We reserve one slide for an FE stain, 
this slide is fixed in Methanol for 10 mins, then continue per usual with 
the FE stain.
Hope this helps!

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net







Debra Siena <DSiena <@t> statlab.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/02/2011 10:49 AM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] bone marrow aspirations






Hi All,

I would appreciate some insight as to how most labs are treating bone 
marrow smears, in regards to fixation.  I am not embarrassed to say that 
it has been awhile since I worked with them.  With the smears are most 
labs air drying, methanol (alcohol fixation) or spray fixation (pap 
fixative) or something else?  I do thank you for your help.

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. | McKinney, TX 75071
Direct: 972-436-1010  x229 | Fax: 972-436-1369
dsiena <@t> statlab.com<mailto:maubrey <@t> statlab.com> | www.statlab.com<
http://www.statlab.com/>


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



--------------------------------------------------------------------------
Disclaimer: This electronic message may contain information that is
Proprietary, Confidential, or legally privileged or protected. It
is intended only for the use of the individual(s) and entity named
in the message. If you are not an intended recipient of this
message, please notify the sender immediately and delete the
material from your computer. Do not deliver, distribute or copy
this message and do not disclose its contents or take any action in
reliance on the information it contains.