[Histonet] IHC slides

[Sebree Linda A]

We've held slides post-retrieval in buffer (tris, PBS, etc.) overnight
with no problem. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC slides

So...I have run down slides and done antigen retrieval on my FFPE
slides.  They are currently in antigen retrieval that has come to room
temperature.  I am not going to be able to finish the IHC stains until
tomorrow.  Will it be better to keep the slides in the antigen retrieval
solution at 4 degrees overnight, or put it in buffer at 4 degrees
overnight?  I really don't want to stay here late.

Thanks histo-hotties!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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