[Histonet] New Methylene Blue

[Rene J Buesa]

The only NEW methylene blue formula I know is the one by Higham (1945) and is prepared as follows:
water → 75 mL
95% ethanol → 25 mL
methylene blue → 0.1 g
citric acid → 0.2 g
To use for 3 to 10 minutesRené J.

--- On Tue, 6/28/11, Breeden, Sara <sbreeden <@t> nmda.nmsu.edu> wrote:



From: Breeden, Sara <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] New Methylene Blue
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, June 28, 2011, 2:24 PM


Okay - it's not for me, but I have offered to make up a new solution of
NEW METHYLENE BLUE for our specimen prep area.  I need a "recipe" for
making an aqueous solution used to stain fluid preps from animals
(urines, etc.).  I've checked Lillie, Carson and Sheehan and Google.
Can't seem to find an appropriate Formula.  Help?



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet