[Histonet] Flk2 / Flt3 / CD135 on mouse bone

[Adam .]

Hi all,

One of my fellow graduate students is trying to perform immunofluorescence
on PFA fixed, EDTA decalcified mouse bone for Flk2 (aka Flt3 or CD135). So
far, he hasn't had any luck on either paraffin embedded or frozen sections
using HIER. Has anyone done this successfully? I know the ideal way to
perform IF on bone is using a tape-transfer system, but, alas, we don't
quite have that working yet.

Thanks,
Adam