[Histonet] EDTA decalcification of bones which have been fixed in 70% ethanol

[Rene J Buesa]

Since EDTA is essentially an aqueous solution, I would wash out the 70EthOL, fix in NBF for 24 hours and then place the specimen in EDTA until decalcification is completed.
René J.

--- On Wed, 6/22/11, Orla M Gallagher <o.m.gallagher <@t> sheffield.ac.uk> wrote:


From: Orla M Gallagher <o.m.gallagher <@t> sheffield.ac.uk>
Subject: [Histonet] EDTA decalcification of bones which have been fixed in 70% ethanol
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, June 22, 2011, 7:55 AM


Dear Histonetters,

We would like to decalcify some mouse bones in EDTA pH 7 which have been
received by our lab already fixed directly in 70% ethanol rather than in
formalin or paraformaldehyde.

How would you recommend preparing these bones for decalcification e.g.
whether to post-fix in formalin or to wash out the ethanol before
transferrring to EDTA? I realise that ethanol is not the best fixative to
use, especially as the end user may want to do immunocytochemistry or enzyme
histochemistry using TRAP.

Thanks,
Orla

-- 
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         0114-2713337 (office)
              0114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk

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