[Histonet] RE: PAS

[Tony Henwood]

Mirna,

Lee Luna (Histologic 6(1):77, 1976) suggests that the best control for PAS staining, are the walls of blood vessels. They will show a bright red reaction only if the technique is working well. Glycogen may stain well even with sub-optimal reagents.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com
Sent: Tuesday, 21 June 2011 1:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PAS

Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control??

Thanks

 

Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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