[Histonet] (no subject)

[Rene J Buesa]

Validating an Ab does not involve testing different concentrations, just a known (+) tissue to be evaluated by the pathologist that should compare the results with other tissues already reacted with the same Ab and protocol.
If the pathologist accepts the results, you have your validation.
René J.

From: Dorothy Glass <techmana12 <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, June 15, 2011 11:41 AM
Subject: [Histonet] (no subject)

Dear Histonets, 
How is it possible to validate a antibody on the Ventana Ultra or XT, when the 
antibodys afre prediluted or made into a prep-kit?
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