[Histonet] Paraffin processing and embeding of Chondrocyte pellets.

[Liz Chlipala]

Bret

We have done a bit of work with these types of samples, the first thing
we do is remove the formalin and add eosin to the container for about 5
minutes.  Then we place the pellet (using a plastic disposable pipettor)
in a mesh bag and process on our tissue processor on a cycle that is set
at 7 minutes per station, we also used propar instead of xylene (3
changes).  We used to process by hand but this method works just as
well.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clough,
Bret
Sent: Tuesday, June 14, 2011 8:52 PM
To: Histonet list serv. (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte
pellets.

Hello Everyone,
 I was wondering if anyone has a protocol for paraffin embedding
chondrocyte pellets that they would be willing to share with me? Any
help would be greatly appreciated.

Thanks,
     Bret

Bret Clough
TAMHSC

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