[Histonet] RE: Paraffin processing and embeding of Chondrocyte

[Linn, Marisa]

Hi Bret-
I've dealt with chondrocyte pellets a few times.  It's a pretty half-assed technique, but works pretty well if the pellet is relatively solid...if it's not, maybe someone else can offer a useful suggestion :)
I was taught to use a piece of lens paper; pre-fold it in half several times, and drop the pellet along one of the folds and close the thing up.  If you trap an edge or two of the paper in the cassette lid (or put a sponge in), the paper shouldn't be able to unfold.  When the time comes to embed, keep that paraffin nice and melty.  
For sectioning...check frequently under a scope.   
Good luck,
Marisa

-----Original Message-----

Message: 19
Date: Wed, 15 Jun 2011 02:52:29 +0000
From: "Clough, Bret" <Clough <@t> medicine.tamhsc.edu>
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte
	pellets.
To: "Histonet list serv. (histonet <@t> lists.utsouthwestern.edu)"
	<histonet <@t> lists.utsouthwestern.edu>
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	<E28DEFDB2660F84B8020A8548E2EB89D1587DF31 <@t> BCSMAIL2.ad.tamhsc.edu>
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Hello Everyone,
 I was wondering if anyone has a protocol for paraffin embedding chondrocyte pellets that they would be willing to share with me? Any help would be greatly appreciated.

Thanks,
     Bret

Bret Clough
TAMHSC

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