[Histonet] Ki-67/PHH3 double staining

[Houston, Ronald]

Has anyone had success double staining these two antibodies for IHC? Ki-67 tends to mask most of the PHH3 staining irrespective of which antibody we use first. Individually, both antibodies have clean and crisp localization. I have seen pictures in articles and on-line of results but am far from attaining anything like these.
We are using the Refine DAB and AP Red kits from Leica on the Bonds. Ki-67 is from Leica and PHH3 from Cell Marque

Any help or suggestions welcome
Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
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