[Histonet] sections falling off slides

[Sheila Adey]

I've never worked with goat knees, but we were experiencing lots of PIN4 prostate biopsies floating off during IHC staining with HEIR.
Since we started cutting the prostate bx's at 3 micons, huge difference. They almost never float off. :)
 
> Date: Mon, 16 May 2011 13:23:02 -0700
> From: rjbuesa <@t> yahoo.com
> To: robin_dean <@t> compbio.com; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] sections falling off slides
> CC: 
> 
> Your test subject is one of the more difficult there are.
> Although you may get some background, use some gelatin dissolved in the water bath to try to adhere the sections to the slides. Also try to cut as thin as you can and make sure there is no water left between the sections and the slide.
> Bake at 60ºC for at least 45 minutes before starting the IHC procedure. If you use HIER try to switch to pepsin.
> The least "stress" you add to the sections the better.
> René J.
> 
> From: Robin Dean <robin_dean <@t> compbio.com>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Monday, May 16, 2011 2:07 PM
> Subject: [Histonet] sections falling off slides
> 
> Hi All,
> 
> 
> 
> We are trying to do IHC for multiple antigens on goat knees. The sections
> are decalcified FFPE sections and we are using a Dako autostainer. We are
> currently trying air-drying for  1-2 days  and baking of tissue slides
> before use in IHC..
> 
> The one previous time we tried baking, we noticed a decrease in IHC signal.
> The sections are pretty large, we tried cutting them down, but can't
> decrease the size much.  We also  decreased the number of washes and size of
> washes on the Daok, which helped only slightly.  Does anyone have any
> suggestions?
> 
> 
> 
> Thank you in advance.
> 
> 
> 
> Robin
> 
> 
> 
> Robin R. Dean, Ph.D.
> 
> Senior Scientist & Study Director
> 
> Comparative Biosciences, Inc.
> 
> 786 Lucerne Dr.
> 
> Sunnyvale, CA
> 
> (408) 738-8060
> 
> robin_dean <@t> compbio.com
> 
> 
> 
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