[Histonet] Processing schedule for fatty tissues

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Jun 10 15:36:40 CDT 2011


The best approach is to increase the fixation time to make sure that the tissues are really fixed.
Then cut the dehydration times of the lower alcohols by 20% and add that 20% of saved time as increase in the clearing agent (supposedly xylene or alkane substitute) times.
René J.

From: Sheila Adey <sadey <@t> hotmail.ca>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Friday, June 10, 2011 4:22 PM
Subject: [Histonet] Processing schedule for fatty tissues


Hello Everyone:

I am looking for a processing schedule for fatty specimens. 

Could anyone share theirs?

Thanks in Advance. 
:) 
Sheila
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