[Histonet] BRDU

C B clb1158 <@t> yahoo.com
Wed Jun 8 19:36:54 CDT 2011


Is anyone using ph6 HEIR instead of HCl/enzyme digestion for BRDU staining?  
I've read 2 manufacturer datasheets that recommend pH 6 HEIR.  I thought the 
routine pretreatment was HCl/enzyme digestion.  We have FFPE rat intestine to 
stain. Any feeback is appreciated.
Cindy Baranowski, HT
SJTRI
Atlanta, GA


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