[Histonet] why no replay????

[Geoff McAuliffe]

Another possibility is that not every goblet cell is making the same 
products at the same time.

Geoff


On 6/4/2011 12:00 PM, mohamed abd el razik wrote:
> thanx to all kind replays from sgoebel, Paula Pierce and JR R.
> but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain sulfated one. my quistion is:  i found in several serial section on intestine that 100 goblet cell react to AB ph 1 and only 50 cell reacts to AB 2.5 why?????? it should be the reverse numbers as at pH 2.5 all acidic sulfated or non sulfated are stained. in other words, ph 2.5 should stain 100 cell as ph 1 stains 100 cell at least. so i'm confused about that and replay or staining several times according to Bancroft and Farida and these was confirmed
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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