[Histonet] why no replay??--Alcian Blue

[JR R]

According to Dako, 



At pH 2.5, Alcian Blue stains sulphated and non-sulphated
acidic carbohydrates. At pH 1.0, only sulphated carbohydrates are stained.
Jerry RicksResearch Scientist 
University of WashingtonDepartment of Pathology



> Date: Fri, 3 Jun 2011 12:21:31 -0700
> From: k84as <@t> yahoo.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] why no replay??
> 
> i have posted my quistion 2-3 times several days ago and recieve no negative or positive comment !!! why?? 
> is my quistion not clear or what??
> 
> --- On Thu, 6/2/11, mohamed abd el razik <k84as <@t> yahoo.com> wrote:
> 
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> From: mohamed abd el razik <k84as <@t> yahoo.com>
> Subject: Alcian blue quistion
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, June 2, 2011, 10:53 AM
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> hi every body
> i have a confusing quistion reagrding Alcian blue pH 1 & 2.5 stains.
> suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell only react to pH 2.5 althogh the other non reacted cells possess acidic sulfated type?? on other word i need to know for what type of mucins alcian B 2.5 react with?
>  
> thanx all
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