[Histonet] nuclear haziness

[Jean Brinker]

Thanks for your response. I had that thought as well, and the techs have 
adjusted their technique to make sure excess water is drained from slides before 
they are placed in the oven. They check them again before staining. 





________________________________
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
To: Jean Brinker <j.brinker <@t> att.net>; "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, June 2, 2011 9:50:08 AM
Subject: Re: [Histonet] nuclear haziness


Make sure that ALL your slides are properly dried in the oven and that NONE has 
any remnant of water underneath before going into the oven to dry.
René J.

From: Jean Brinker <j.brinker <@t> att.net>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, June 2, 2011 6:03 AM
Subject: [Histonet] nuclear haziness

We are having an issue with nuclear haziness on prostate biopsies in our small 
urology laboratory.  It occurs randomly, appearing on some levels and not others 

within the same block. Another scenario is that we sometimes see it occur only 
in one small area of a core and the remaining tissue in the core looks 
beautiful. It can occur throughout a case (12 blocks) or not at all. We have 
checked for obvious problems, i.e. reagent contamination on the processor and 
stainer, ruled out temperature issues, microtome problems, etc. to no avail. I 
would appreciate any input/suggestions that would help to alleviate this 
problem. 

Thank you,

Jean Brinker
St. Louis Urological Surgeons
St. Peters, MO
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