[Histonet] Lipofuscin and counterstaining DAb IHC

[szigcs <@t> bio.u-szeged.hu]

Dear Histonetters!

I have human brain samples (age 70, HUntington disease) and my goal is  
to quantitate changes in the number of neurons/glia cells with nuclei  
staining.
I use NeuN IHC and Höechst. My first question is:
  - Throughout the whole sample there are a lot of lipofuscin signal  
fading the NeuN signal, we are not able to make any microphtograph for  
cell counting.
I tried Sudan black staining and Sigma autofluorescent reagent with  
the same result. Could you advise some tips and tricks to avoid this?

An alternative solution could be DAB IHC for NeuN and  counterstaining  
with nucleus staining.
Second question is:
- what type of counterstain do you recommend to be able to identify  
the IHC and normal nucleus staining in the same section?

Thank you in advance, best regards
Csaba





----------------------------------------------------------------
This message was sent using IMP, the Internet Messaging Program.