[Histonet] Melanoma sentinel nodes

[Orr, Rebecca]

Amy,
I can't find the exact reference but I think either Balch or Taylor authored the paper that was adapted for our protocol.

Level 1, 3, 5, 10 are H/E and extra section at level 5 is taken for Negative serum control
Level 2 is S100
Level 4, 6 is PanMelanoma cocktail (Biocare Medical)
Level 7, 8, 9, are held for  as unstained and filed away additional H/E or IHC

We usually aim for about 200 microns in between  levels.  This is entirely dependent on the degree of adipose tissue that was included around the node and the thickness of the node in the paraffin block.

When we started this protocol  about 5 years ago, we would get 1 or 2 paraffin blocks for analysis.
Nowadays we typically receive 3-6 blocks per case (sometimes 2 cases per week) and this has impacted our workload.

Hope this helps.

Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771


From: "Amy Farnan" <Farnana <@t> nehealth.com>
Subject: [Histonet] sentinel lymph node melanoma protocols
To: <histonet <@t> lists.utsouthwestern.edu>
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Good morning everyone,

I am curious to see what the standard protocol is on sentinel lymph node for melanomas.
I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple H&E sections and IHC in between to bivalving.  Some of the protocols call for up to as many as 46 H&E sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail).  Would any of you like to share your protocols?

thank you-
Amy Farnan
Histology Supervisor
Northeast Health
Albany, New York

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Message: 7
Date: Wed, 1 Jun 2011 09:40:03 -0700 (PDT)
From: Dawud AlYasa <dawudhill <@t> yahoo.com>
Subject: [Histonet] Bone Marrow H&E Protocols
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <759774.67432.qm <@t> web34506.mail.mud.yahoo.com>
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Hello Histonet!
?
Does anyone have an H&E automated stainer protocol for bone marrows that they wouldn't mind sharing? I'm looking at different protocols. It would be greatly appreciated. Thanks.
?
Dawud

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Message: 8
Date: Wed, 1 Jun 2011 09:54:01 -0700
From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
Subject: [Histonet] pic of tattooed skin
To: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <55DCEF43-7DD7-4117-A62D-5790DA604224 <@t> email.arizona.edu>
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Good Morning!
Found out a few days ago that next week we are participating in a summer camp for high schoolers and it was decided to use skin this year for our examples. I have lots of slides of skin sections from mice, rats, frogs, etc. and even some human skin punches - normal and with skin cancers from when we did a project for a sunscreen manufacturer. What I was looking for was an example of tattooed skin and I spent a bit of time searching for one yesterday online but couldn't find anything that was suitable. Does anyone out there have a picture of skin that has been tattooed? And maybe a good skin scar?
Last year's camp was on liver, which was way easier!

Thanks.
Andi Grantham

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End of Histonet Digest, Vol 91, Issue 1
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