[Histonet] RE: Cresyl violet stain on 50 um mouse brain sections
Entwistle, Laura
lentwistle <@t> ucsd.edu
Fri Jul 29 10:13:25 CDT 2011
We have done the same, but also adding a 50 and 70 ETOH as well. Our slides are also gel coated. We also have them in a 1 to 1 alcohol/chloroform, but only have them in for 30 minutes.
Laura
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael, Susan
Sent: Friday, July 29, 2011 6:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections
I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions?
Susan
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