[Histonet] What is 10% Buffered Formalin Acetate

[Geoff McAuliffe]

More likely buffered with calcium acetate.
The late Ralph Lillie advocated 2% calcium acetate as a good buffer for 
formalin mixtures.
One gets buffering plus some calcium ions to help with the stabilization 
of lipids.Also easy, just one weighing.
Anything Lillie recommends is on a very sound scientific footing.

Geoff

On 7/28/2011 11:28 AM, Harvey, Jennifer Lynn wrote:
> I had a investigator say that he is using 10% Buffered Formalin Acetate to fix his mouse brains. They order it from Fisher (127-09-3). It is buffered with Sodium Acetate instead. Has anyone ever used this and why would you want to? I have never heard of it before! It is a new one to me!
>
> Jennifer Harvey, HT(ASCP) QIHC
> Vanderbilt University Medical Center
> Neuropathology Lab Supervisor
> C-2309 Medical Center North
> Nashville, TN  37232-2561
> Phone: 615-343-0083
> Fax: 615-343-7089
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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