[Histonet] Biocare decloaker

[Blazek, Linda]

I just sizzle.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles Claire 
Sent: Wednesday, July 27, 2011 3:17 PM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Biocare decloaker

I swear I blow a gasket at least once a week!   Oh wait that's me, not the decloker. :)
Claire

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Weems, Joyce
Sent: Mon 7/25/2011 3:42 PM
To: William; Mike Pence
Cc: <histonet <@t> lists.utsouthwestern.edu>; Diana McCaig
Subject: RE: [Histonet] Biocare decloaker



I wonder if a new gasket would help me.. I keep blowing mine! :>)


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of William
Sent: Monday, July 25, 2011 16:40
To: Mike Pence
Cc: <histonet <@t> lists.utsouthwestern.edu>; Diana McCaig
Subject: Re: [Histonet] Biocare decloaker

As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds.

My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months.

Will Chappell

Sent from my iPhone

On Jul 25, 2011, at 1:34 PM, "Mike Pence" <mpence <@t> grhs.net> wrote:

> There is a huge difference between 122 degrees for 30 seconds and 90
> degrees for 45 minutes. I would say you need to look at something here!
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
> McCaig
> Sent: Monday, July 25, 2011 2:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Biocare decloaker
>
>
> We are trying to validate a Biocare decloaker and have found when we
> use
> 122 degrees for 30 seconds we get great signal, but distorted
> morphology.
>
>
>
> If we reduce to 90 degrees for  45 minutes, the signal is
> significantly decreased but the morphology is good.
>
>
>
> What protocols are you using?
>
>
>
> Diana
>
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