[Histonet] RE: Histonet Digest, Vol 92, Issue 33

[Joanne Clark]

This sounds to me like a processing issue, especially with the types of tissues you describe.  Are the tissue sections you are referring to very thick?  Maybe the tissue is too big for the amount of time in the different processing stations.  I have also seen this happen when the tissue has been left to dry out before the clinician puts it into the formalin.

Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico

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Message: 2
Date: Mon, 25 Jul 2011 13:42:25 -0400
From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: [Histonet] blurry tissue 
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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I would like some thoughts on how to resolve some "blurry" looking tissue.  We have had occasional tissue that looks blurry and not crisp for several weeks now.  It is not all the cases only random tissues.  The tissue is not on the same tissue processor either. We have 2 processors.  The latest cases were a breast, some skins, and a prostate.  I am not certain if this is happening on the tissue processor or in the stainer.  It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene.  It is so hit and miss that I am puzzled.   Any suggestions would be greatly appreciated.
Thank you in advance for your thoughts.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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