[Histonet] Biocare decloaker

[William]

Ok that is a bigger problem and a very clear answer to your staining issue. Your decloaker should never "blow" a gasket. Something is wrong and you probably need service or a replacement. Your decloaker is probably getting too hot and over retrieving tissue.  I suggest immediately calling Biocare technical support. 

You need this fixed!

Will Chappell


Sent from my iPhone

On Jul 25, 2011, at 1:42 PM, "Weems, Joyce" <JWeems <@t> sjha.org> wrote:

> I wonder if a new gasket would help me.. I keep blowing mine! :>) 
> 
> 
> Joyce Weems 
> Pathology Manager 
> Saint Joseph's Hospital 
> 5665 Peachtree Dunwoody Rd NE 
> Atlanta, GA 30342 
> 678-843-7376 - Phone 
> 678-843-7831 - Fax 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of William
> Sent: Monday, July 25, 2011 16:40
> To: Mike Pence
> Cc: <histonet <@t> lists.utsouthwestern.edu>; Diana McCaig
> Subject: Re: [Histonet] Biocare decloaker
> 
> As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. 
> 
> My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. 
> 
> Will Chappell
> 
> Sent from my iPhone
> 
> On Jul 25, 2011, at 1:34 PM, "Mike Pence" <mpence <@t> grhs.net> wrote:
> 
>> There is a huge difference between 122 degrees for 30 seconds and 90 
>> degrees for 45 minutes. I would say you need to look at something here!
>> 
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana 
>> McCaig
>> Sent: Monday, July 25, 2011 2:45 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Biocare decloaker
>> 
>> 
>> We are trying to validate a Biocare decloaker and have found when we 
>> use
>> 122 degrees for 30 seconds we get great signal, but distorted 
>> morphology.
>> 
>> 
>> 
>> If we reduce to 90 degrees for  45 minutes, the signal is 
>> significantly decreased but the morphology is good.
>> 
>> 
>> 
>> What protocols are you using?
>> 
>> 
>> 
>> Diana
>> 
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>> 
>> 
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