[Histonet] Biocare decloaker

[William]

As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. 

My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. 

Will Chappell

Sent from my iPhone

On Jul 25, 2011, at 1:34 PM, "Mike Pence" <mpence <@t> grhs.net> wrote:

> There is a huge difference between 122 degrees for 30 seconds and 90
> degrees for 45 minutes. I would say you need to look at something here!
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
> McCaig
> Sent: Monday, July 25, 2011 2:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Biocare decloaker
> 
> 
> We are trying to validate a Biocare decloaker and have found when we use
> 122 degrees for 30 seconds we get great signal, but distorted
> morphology.
> 
> 
> 
> If we reduce to 90 degrees for  45 minutes, the signal is significantly
> decreased but the morphology is good.
> 
> 
> 
> What protocols are you using?
> 
> 
> 
> Diana
> 
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