[Histonet] H&E protocol question

[Rene J Buesa]

Progressive hematoxylins usually take more time to stain  and for routine work the schedule has to be very "fine tuned" because otherwise obtaining the ideal staining is quite difficult with a frequent tendency to the "under staining".
Regressive hematoxylins on the other hand, always over stain and then you can obtain the ideal staining by removing the hematoxylin with acid or aqueous solutions. They are faster and the results are more easy to control.
For many years I always prepared my hematoxylins, but now there are commercial solutions of great quality and consistent results so it is better (although no more cost effective) to buy a reputable brand, develop a good protocol and stick to it. At the end I relied on Richard Allen hematoxylins.
Eosin, on the other hand, are so easy to prepare that I always prepared mine.
René J.

--- On Mon, 7/25/11, histotech <@t> imagesbyhopper.com <histotech <@t> imagesbyhopper.com> wrote:


From: histotech <@t> imagesbyhopper.com <histotech <@t> imagesbyhopper.com>
Subject: [Histonet] H&E protocol question
To: "Histonet <@t> Lists. Utsouthwestern. Edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, July 25, 2011, 7:29 AM


Hi Histonetters!

I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive H&E on routine daily work.

Which hematoxylins do you prefer (commercially prepared), which eosin?

Anyone have a tried and true protocol for each method?

Thanks!

Michelle

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