[Histonet] RE: Frozen eye problems

[Anatoli Gleiberman]

Try Neg-50 (Fisher, cat#22-110-617) instead of OCT, we don't have any problem with fresh-frozen mouse eyes.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Boyce, Bobbie
Sent: Monday, July 25, 2011 10:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen eye problems

Hello Histoneters,

I'm having problems getting a good section on paraformaldyehyde fixed
adult mouse eyes frozen in TBS tissue freezing medium. One of the
problems was the orentation of them. They were too close to the edge. I
also, wanted to try a harder freezing medium, so I corrected oriented
and used an OCT equivalent tissue freezing medium.  They looked better,
but still not as nice as I think they should be.

My eye contact tells me that they should be cut at -20 for adult and -14
for neonatal. They have me set the knife angle at 0 to -2.5. 

I also tried cutting these as I would cut any other frozen issue, but
I'm just pulling my hair out.

Does anyone have any other suggestions that I might try? Your help would
be greatly appreciated.

Bobbie Boyce
Histology Specialist III
duPont Hospital for Children
Wilmington, DE
302-651-6771 (Lab)
302-651-5010 (Fax)





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