[Histonet] Fast Blue stabilization

[carpj]

Hi List,

We are retrogradely labeling spinal motoneurons with Fast Blue, which works well. When we process our sections (60-µm) for immunohistochemical labeling, the Fast Blue signal becomes much weaker. We believe that the detergents we are using to facilitate antibody penetration are also allowing the Fast Blue to leak out of the cells. Is anyone aware of methods for stabilizing Fast Blue within the cells so it won't wash out during immunohistochemical processing?

Jonathan Carp, Ph.D.
Wadsworth Center
New York State Dept. Health
Albany, NY




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