[Histonet] Research microtomes

[Jack Ratliff]

Thanks a lot for your response Adrienne! I think I may be able to help you with this. Is there a time we can talk off-line over the phone? My number is 317-281-1975.

Best Regards,

Jack



On Jul 21, 2011, at 9:49 AM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:

> This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing:
> 
> "In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth.
> 
> Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns."
> 
> Thanks,
> Adrienne
> 
> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
> To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
> Sent: Wednesday, July 20, 2011 6:13 PM
> Subject: Re: [Histonet] Research microtomes
> 
> Couple more questions. :) What is the tissue and the dimensions of the specimen?
> 
> 
> 
> On Jul 20, 2011, at 4:57 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
> 
>> Hi Jack,
>> 
>> We're trying to cut just plain old FFPE blocks.
>> 
>> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
>> To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
>> Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
>> Sent: Wednesday, July 20, 2011 5:02 PM
>> Subject: Re: [Histonet] Research microtomes
>> 
>> What type of specimen are you trying to cut? What embedding media are you using?
>> 
>> Jack
>> 
>> 
>> 
>> On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
>> 
>> > Hello Histo-land,
>> > 
>> > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated!
>> > 
>> > Thanks,
>> > Adrienne
>> > _______________________________________________
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>> > Histonet <@t> lists.utsouthwestern.edu
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> > 
>> 
>> 
> 
>