[Histonet] RE: Leica Service Technician

Suresch, Donna L. donna_suresch <@t> merck.com
Wed Jul 20 13:16:40 CDT 2011


Hello Amanda,
We are located in Eastern Pa and we use Belair Instruments out of NJ (908-518-2662).  We have been very happy with their service on our cryostats.



Donna L. Suresch
Merck Research Laboratories
Research Biologist
Imaging Research - West Point


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Subject: Histonet Digest, Vol 92, Issue 27

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Today's Topics:

   1. Leica Service Technician (Amanda Madden)
   2. Controls with patient specimen on same slide (SHargrove <@t> urhcs.org)
   3. please remove me from the list, thank you! (Robert Cordero)
   4. RE: Leica Service Technician (Sherwood, Margaret )
   5. Cat Scratch control (Houston, Ronald)
   6. RE: Leica Service Technician (Tom McNemar)
   7. RE: Controls with patient specimen on same slide (Tom McNemar)
   8. RE: Assisting with Autopsies  (Weems, Joyce)
   9. Controls with patient specimen on same slide (Nancy Schmitt)


----------------------------------------------------------------------

Message: 1
Date: Wed, 20 Jul 2011 11:32:07 -0400
From: Amanda Madden <amkmadden <@t> gmail.com>
Subject: [Histonet] Leica Service Technician
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CAC=+zgXtEK9=c0_nUSddHcSp4_7SDsn2jFxT3kYCJh=NSwUH0A <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi Histonetters!

A few months back I emailed regarding a service contract through Leica for
our CM 3050S, and (unfortunately?) we chose not to purchase one. This week
we have had a serious issue with it... the specimen head and arm is covered
in frost, and the object temperature sensor is reading ## instead of a temp.
In any case, we called Leica and asked for a service call, but it is
extremely expensive and they couldn't give us an estimate of when they will
be here because cryostats used for clinical applications have priority over
those, like ours, that are used for research. Understandable, but
frustrating nonetheless. So my question is: does anyone know of a good,
reputable cryostat service technician (who is authorized by leica, if
possible) that is located in the Boston, MA area?

Any help would be greatly appreciated!

Thanks in advance,
Amanda


------------------------------

Message: 2
Date: Wed, 20 Jul 2011 10:35:33 -0500
From: SHargrove <@t> urhcs.org
Subject: [Histonet] Controls with patient specimen on same slide
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <OFE0363896.5E031032-ON862578D3.005508B0-862578D3.0055A776 <@t> urhcs.org>
Content-Type: text/plain; charset="us-ascii"

In our Lab we put a control on every slide. We keep multiple controls cut
and stored in refrigerator. Most are multi- tissue controls. We use the
Ventana detection kits and it is not cost efficient to use 2 uses of the
detection kit per antibody.  Of course there are times  that this will not
work, then our cost per test goes up, but we cannot charge any more for a
control.
(Embedded image moved to file: pic32000.jpg)

------------------------------

Message: 3
Date: Wed, 20 Jul 2011 15:37:44 +0000
From: Robert Cordero <Robert.Cordero <@t> comphealth.com>
Subject: [Histonet] please remove me from the list, thank you!
To: "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <0BA36F96367E8A4CBB27E112FAA6488A0679E9 <@t> vslcexmbp02.mychg.com>
Content-Type: text/plain; charset="us-ascii"





------------------------------

Message: 4
Date: Wed, 20 Jul 2011 11:43:46 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] Leica Service Technician
To: "Amanda Madden" <amkmadden <@t> gmail.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <073AE2BEA1C2BA4A8837AB6C4B943D9708DB58D3 <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"

Amanda,

We have a Microm HM550 cryostat and have used Brian J. Hurley (New England
Biomedical Services).  He is an independent service engineer.  He's very good.
In fact ThermoFisher uses him if a service visit is needed.

781-331-8642
617-774-7368 (cell)

Peggy


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amanda Madden
Sent: Wednesday, July 20, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Service Technician

Hi Histonetters!

A few months back I emailed regarding a service contract through Leica for
our CM 3050S, and (unfortunately?) we chose not to purchase one. This week
we have had a serious issue with it... the specimen head and arm is covered
in frost, and the object temperature sensor is reading ## instead of a temp.
In any case, we called Leica and asked for a service call, but it is
extremely expensive and they couldn't give us an estimate of when they will
be here because cryostats used for clinical applications have priority over
those, like ours, that are used for research. Understandable, but
frustrating nonetheless. So my question is: does anyone know of a good,
reputable cryostat service technician (who is authorized by leica, if
possible) that is located in the Boston, MA area?

Any help would be greatly appreciated!

Thanks in advance,
Amanda
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Message: 5
Date: Wed, 20 Jul 2011 15:55:50 +0000
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] Cat Scratch control
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>,    "ihcrg <@t> googlegroups.com"
        <ihcrg <@t> googlegroups.com>
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        <E5D0CD2352E46545A0C0EBE308CCCE530BC6D1 <@t> L1PERDWXMB01.childrensroot.net>

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Does anyone have an extra Cat Scratch control block to spare? We currently buy control sections from Newcomer, but I'm not convinced they are that good.
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.NationwideChildrens.org>

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Message: 6
Date: Wed, 20 Jul 2011 12:26:11 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] Leica Service Technician
To: 'Amanda Madden' <amkmadden <@t> gmail.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
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        <E9A90E28259D2F4E84308C5E8EA8F7B4381967AD20 <@t> lmhs-exchange.lmhealth.org>

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It is really just a freezer with a microtome in it.  Do you have an on-site refrigeration guy that could look at it?  I have used our in-house guy a time or two.  Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amanda Madden
Sent: Wednesday, July 20, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Service Technician

Hi Histonetters!

A few months back I emailed regarding a service contract through Leica for
our CM 3050S, and (unfortunately?) we chose not to purchase one. This week
we have had a serious issue with it... the specimen head and arm is covered
in frost, and the object temperature sensor is reading ## instead of a temp.
In any case, we called Leica and asked for a service call, but it is
extremely expensive and they couldn't give us an estimate of when they will
be here because cryostats used for clinical applications have priority over
those, like ours, that are used for research. Understandable, but
frustrating nonetheless. So my question is: does anyone know of a good,
reputable cryostat service technician (who is authorized by leica, if
possible) that is located in the Boston, MA area?

Any help would be greatly appreciated!

Thanks in advance,
Amanda
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.



------------------------------

Message: 7
Date: Wed, 20 Jul 2011 12:30:06 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] Controls with patient specimen on same slide
To: "'Rathborne, Toni'" <trathborne <@t> somerset-healthcare.com>, 'Richard
        Cartun' <Rcartun <@t> harthosp.org>, "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E9A90E28259D2F4E84308C5E8EA8F7B4381967AD21 <@t> lmhs-exchange.lmhealth.org>

Content-Type: text/plain; charset="us-ascii"

Each case gets one positive control per antibody.  If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide

All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides?

-----Original Message-----
From: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
Sent: Tuesday, July 19, 2011 6:40 PM
To: histonet <@t> lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide

We do not put our positive control tissue on the test slide; we run batch controls.  Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol.  For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC.  Therefore, it would be very difficult for us to place the positive control tissue on the same slide.  In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing.  Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive.  However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 7/19/2011
>>> 3:27 PM >>>

Hi,
I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this?  If you're not, why not?
Thanks,
Toni


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This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.



------------------------------

Message: 8
Date: Wed, 20 Jul 2011 12:53:21 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Assisting with Autopsies
To: Amy Self <ASelf <@t> georgetownhospitalsystem.org>,
        "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <92AD9B20A6C38C4587A9FEBE3A30E164082122A73D <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

We have two contract deiners.. They work at other hospitals around town and charge per case. Works well here as our numbers have decreased drastically over the years.


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Tuesday, July 19, 2011 15:12
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Assisting with Autopsies

Hello All,

We are small hospital that does approximately 5-10 autopsies a year.  This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll.

Does your facility use histotechs or a diener to assist with the autopsy?


Thanks in advance for all of your help, Amy


Amy Self
Georgetown Hospital System
843-527-7179
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Message: 9
Date: Wed, 20 Jul 2011 16:59:53 +0000
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
Subject: [Histonet] Controls with patient specimen on same slide
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
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        <906B4DA90ED1DB4DB6C7E94D7CEE6C36791511 <@t> PEITHA.wad.pa-ucl.com>
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We place control tissue on EACH slide for IHC testing - even if multiple blocks from same case.

Nancy Schmitt HT, MLT(ASCP)
United Clinical Laboratories

-----------------------------------------

Message: 12
Date: Wed, 20 Jul 2011 13:18:52 +0000
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] Controls with patient specimen on same slide
To: "'Richard Cartun'" <Rcartun <@t> harthosp.org>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <3AD061FE740D464FAC7BF6B5CFB75707031366 <@t> SMCMAIL01.somerset-healthcare.com>

Content-Type: text/plain; charset="us-ascii"

All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides?



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