[Histonet] Controls with patient specimen on same slide

[Sebree Linda A]

Just one. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Wednesday, July 20, 2011 8:19 AM
To: 'Richard Cartun'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide

All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks
for a case, or just on one of the slides?

-----Original Message-----
From: Richard Cartun [mailto:Rcartun <@t> harthosp.org] 
Sent: Tuesday, July 19, 2011 6:40 PM
To: histonet <@t> lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide

We do not put our positive control tissue on the test slide; we run
batch controls.  Many of the unstained slides (breast, GI, and prostate
biopsies) that we use for IHC testing are cut in our Histology
Laboratory as part of a part-type slide protocol.  For example, we cut 7
slides, 2 sections on each slide, for breast biopsies and stain #1, 4,
and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC.
Therefore, it would be very difficult for us to place the positive
control tissue on the same slide.  In addition, I receive a lot of
consult cases from other hospitals where they send us unstained slides
for testing.  Once again, it would be difficult to place the positive
control tissue on the same slide and it would slow us down in terms of
starting those slides once they arrive.  However, I think the main
reason we don't pursue putting the positive control tissue on the same
slide is the fact that it would consume an enormous amount of control
tissue.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 7/19/2011 
>>> 3:27 PM >>>

Hi,
I'm interested in knowing how many of you are performing ihc with the
control tissue and the patient tissue on the same slide. I have seen
slides available which have designated areas for each tissue to be
placed so there will not be any confusion. If you're doing it, have you
encountered any problems? What benefits have you noticed since
implementing this process? Are your pathologists in favor of this?  If
you're not, why not?
Thanks,
Toni


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and/or
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forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.

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